LOCUS       sequence-160458-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV H-Ras V12 IRES GFP
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(117..629,630..632,633..635))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     310..329
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     569..590
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(809..828)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
       61 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
      121 atgttgtggc ggatcttgaa gttcgccttg atgccgttct tctgcttgtc ggccatgata
      181 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      241 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      301 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      361 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
      421 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      481 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      541 ccagacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      601 accccggtga acagctcctc gcccttgctc accatggttg tggccatatt atcatcgtgt
      661 ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga
      721 ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac
      781 cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc
      841 cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta
      901 tacacgtggc ttttggccgc agaggcacct gtcgccaggt gggg
//
