LOCUS       sequence-160466-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of YFP 
            and DHFR-TS selectable marker cassette for endogenous tagging on the
            gene locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pYFP.LIC.DHFR
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(88..90,91..93,94..807)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGVDELYS"
     primer_bind     complement(133..154)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(394..413)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     741..762
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccctgt acttccaatc caatttaatt
       61 aaaattggaa gtggaggacg ggaattcatg gtgagcaagg gcgaggagct gttcaccggg
      121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
      301 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      541 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      601 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      661 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
      721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      781 ctcggcgtgg acgagctgta cagttaaggc gcgccagaag ctgcccgtct ctcgttttcc
      841 tctcttttcg gagggatcag ggagagtgcc tcgggtcgga gagagctgac gagggggtgc
      901 cagagacccc tgtgtccttt atcgaagaaa agggatgact cttcatgtgg catttcacac
      961 agtctcacct cgccttgttt tctttttgtc aatcagaacg aaagc
//
