LOCUS       sequence-160467-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of YFP 
            and DHFR-TS selectable marker cassette for endogenous tagging on the
            gene locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pYFP.LIC.DHFR
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     794..810
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     795..811
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagctgtaca gttaaggcgc gccagaagct gcccgtctct cgttttcctc tcttttcgga
       61 gggatcaggg agagtgcctc gggtcggaga gagctgacga gggggtgcca gagacccctg
      121 tgtcctttat cgaagaaaag ggatgactct tcatgtggca tttcacacag tctcacctcg
      181 ccttgttttc tttttgtcaa tcagaacgaa agcgagttgc gggtgacgca gatgtgcgtg
      241 tatccactcg tgaatgcgtt atcgttctgt atgccgctag agtgctggac tgttgctgtc
      301 tgcccacgac agcagacaac tttccttcta tgcacttgca ggatggtgca gcgcaaacga
      361 cggagagaaa ggagcaccct ctcagtttcc ctacgatgtg ctgtcagttt cgactcttca
      421 ccgcgaacga ttggcgatac gtctctgttg acttgttagg ctccgaccac gaagctccct
      481 taactagata agccgcgaca cctaagtgta caccatttgc agatcgataa tctgcgaccg
      541 ctgaatccgt ccagatcagt aaaaccgcac cacctaagtg taaaccttgt ttaggtcgat
      601 aaaatgctac caacccccac ccacaatcga gccttgagcg tttctgcgca cgcgttggcc
      661 tacgtgactt gctgatgcct gcctctggcc attcatgcca gtcagtgcgc ataaaaatgt
      721 ggacacagtc ggttgacaag tgttctggca ggctacagtg acaccgcggt ggagggggat
      781 ccactagttc tactcgaggt cgacggtatc gataagcttc gccaggctgt aaatcccgtg
      841 agtcgtcctc acaaatcatc aagcaggtgt cctcagggag actgcctgac tgagttatgc
      901 taattccttt ctactttgg
//
