LOCUS       sequence-160470-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses E. coli succinyl-CoA synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  TITLE     pSucCD2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSucCD2
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat atacatatga acttacatga atatcaggca
      181 aaacaactgt ttgcccgcta tggcttacca gcaccggtgg gttatgcctg tactactccg
      241 cgcgaagcag aagaagccgc ttcaaaaatc ggtgccggtc cgtgggtagt gaaatgtcag
      301 gttcacgcag gcggccgcgg taaagcgggc ggtgtgaaag ttgtaaacag caaagaagac
      361 atccgtgctt ttgcagaaaa ctggctgggc aagcgtctgg taacgtatca aacagatgcc
      421 aatggccaac cggttaacca gattctggtt gaagcagcga ccgatatcgc taaagagctg
      481 tatctcggtg ccgttgtaga ccgtagttca cgtcgcgtgg tctttatggc ctccaccgaa
      541 ggcggcgtgg aaatcgaaaa agtggcggaa gaaactccgc acctgatcca caaagttgcg
      601 cttgatccgc tgactggccc gatgccgtat cagggacgcg agctggcgtt caaactgggt
      661 ctggaaggta aactggttca gcagttcacc aaaatcttca tgggcctggc gaccattttc
      721 ctggagcgcg acctggcgct gatcgaaatc aacccgctgg tcatcaccaa acagggcgat
      781 ctgatttgcc tcgacggcaa actgggcgct gatggcaacg ccctgttccg ccagcctgat
      841 ctgcgcgaaa tgcgtgacca gtcgcaggaa gatccgcgtg aagcacaggc tgcacagtgg
      901 gaactgaact acgttgcgct ggacggtaac atcggttgta tggttaacgg cgcaggcctg
      961 gcgatgggta cgatggacat cgttaaactg cacggcggcg aaccggctaa cttcctggac
     1021 gttggcggcg gcgca
//
