LOCUS       sequence-160477-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type MscL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Heureaux J, Chen D, Murray VL, Deng CX, Liu AP
  TITLE     Activation of a bacterial mechanosensitive channel in mammalian 
            cells by cytoskeletal stress.
  JOURNAL   Cell Mol Bioeng. 2014 Sep;7(3):307-319.
  PUBMED    25606062
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Bioeng."; date: "2014-09"; volume: "7(3)"; pages: "307-319"
COMMENT     Sequence Label: pADtet-His6-MscL
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..91
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             134..151
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(618..634)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(618..634)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(671..689)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    690..824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(727..746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     781..800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    833..866
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgccac
       61 catgagagga tctcaccatc accatcacca tacggatccg catgcgagct cggtaccccg
      121 ggtcgacctg cagctggttc cgcgtggatc cctcgagcat agggagaata acatgagcat
      181 tattaaagaa tttcgcgaat ttgcgatgcg cgggaacgtg gtggatttgg cggtgggtgt
      241 cattatcggt gcggcattcg ggaagattgt ctcttcactg gttgccgata tcatcatgcc
      301 tcctctgggc ttattaattg gcgggatcga ttttaaacag tttgctgtca cgctacgcga
      361 tgcgcagggg gatatccctg ctgttgtgat gcattacggt gtcttcattc aaaacgtctt
      421 tgattttctg attgtggcct ttgccatctt tatggcgatt aagctaatca acaaactgaa
      481 tcggaaaaaa gaagaaccag cagccgcacc tgcaccaact aaagaagaag tattactgac
      541 agaaattcgt gatttgctga aagagcagaa taaccgctct taatcaggac tcagatccgt
      601 cgacctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
      661 gcttttgttc cctttagtga gggttaatta acttgtttat tgcagcttat aatggttaca
      721 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      781 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcatcgat ccataacttc
      841 gtataatgta tgctatacga agttatccag atctggttct atagtgtcac ctaaa
//
