LOCUS       sequence-160478-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type MscL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Heureaux J, Chen D, Murray VL, Deng CX, Liu AP
  TITLE     Activation of a bacterial mechanosensitive channel in mammalian 
            cells by cytoskeletal stress.
  JOURNAL   Cell Mol Bioeng. 2014 Sep;7(3):307-319.
  PUBMED    25606062
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Bioeng."; date: "2014-09"; volume: "7(3)"; pages: "307-319"
COMMENT     Sequence Label: pADtet-His6-MscL
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     60..76
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     60..76
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(543..560)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(603..620)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(717..741)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(738..776)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    809..827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    851..869
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    893..911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agttaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
       61 ctagaactag tggatccccc gggctgcagg tcgacggatc tgagtcctga ttaagagcgg
      121 ttattctgct ctttcagcaa atcacgaatt tctgtcagta atacttcttc tttagttggt
      181 gcaggtgcgg ctgctggttc ttcttttttc cgattcagtt tgttgattag cttaatcgcc
      241 ataaagatgg caaaggccac aatcagaaaa tcaaagacgt tttgaatgaa gacaccgtaa
      301 tgcatcacaa cagcagggat atccccctgc gcatcgcgta gcgtgacagc aaactgttta
      361 aaatcgatcc cgccaattaa taagcccaga ggaggcatga tgatatcggc aaccagtgaa
      421 gagacaatct tcccgaatgc cgcaccgata atgacaccca ccgccaaatc caccacgttc
      481 ccgcgcatcg caaattcgcg aaattcttta ataatgctca tgttattctc cctatgctcg
      541 agggatccac gcggaaccag ctgcaggtcg acccggggta ccgagctcgc atgcggatcc
      601 gtatggtgat ggtgatggtg agatcctctc atggtggcga attcggggcc gcggaggctg
      661 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
      721 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg cctacctcga
      781 cccgggtacc gagctcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
      841 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
      901 actgataggg agtggtaaac tcgactttca cttttctcta tcactga
//
