LOCUS       sequence-160487-        8492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CRISPR HDAC3 dual gRNA targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8492)
  TITLE     Chen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-L3US2-RFP-HDAC3
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     protein_bind    1384..1483
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1585..1833
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1852..1927
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1960..2208
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2227..2302
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(2343..2438)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2507..2624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2693..2904
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2924..2933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2930..3661
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             3677..3733
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3734..4333
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3734..3753)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4230..4250
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4349..4937
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4402..4422)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4820..4831)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5009..5242
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5320..5441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5357..5376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5411..5430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5481..5616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5636..5655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5637..5655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5665..5682)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5665..5681)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5674..5696)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5823..6278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5910..5929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6120..6141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6304..6408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6409..6477,6478..7269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6627..6646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7440..8028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7929..7948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8182..8199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8316..8337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8352..8357,8358..8375,8376..8382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8390..8406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8395..8417
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8414..8430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8414..8430
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8449..8469
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8451..8469
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctcgttagc ctagaacact caagggagcg
     1381 ctgcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     1441 caatgctttt ttataatgcc aactttgtac aaaaaagcag gctgactcag atagcatgga
     1501 ccagacacta gtaaaatgca tgtccattac tagcatacga cgatacgcct gcaggaaaga
     1561 attccagatg cacggagatg atgcgagggc ctatttccca tgattccttc atatttgcat
     1621 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     1681 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     1741 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     1801 gctttatata tcttgtggaa aggacgaaac accgttctgc tcgcgttaca cgttttagag
     1861 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     1921 tcggtgcttt tttgaccaac gagcaagata aggactaggg agggcctatt tcccatgatt
     1981 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     2041 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2101 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2161 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcaatgcca
     2221 ggcgatgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     2281 aaaagtggca ccgagtcggt gctttttttt ggtaccccac atactgcttc cagacctcta
     2341 gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     2401 cgaacaggtc actatcagtc aaaataaaat cattattttc tagaagcgct acagtcgcct
     2461 catgttagtg gaagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2521 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2581 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2641 acagcagaga tccactttgg cgccggctcg agtggctccg gtgcccgtca gtgggcagag
     2701 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2761 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2821 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2881 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggtgtctaa
     2941 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
     3001 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     3061 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     3121 cagcttcatg tacggcagca gaaccttcat caaccacacc cagggcatcc ccgatttctt
     3181 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     3241 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     3301 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     3361 ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaaccgacat
     3421 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc
     3481 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga
     3541 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
     3601 atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa
     3661 ggctagcggc agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga
     3721 gaaccccggc cccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3781 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3841 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3901 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgcggccg tggcggtctg
     3961 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4021 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4081 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     4141 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4201 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4261 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4321 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     4381 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4441 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4501 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4561 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4621 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4681 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4741 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4801 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4861 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4921 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     4981 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5221 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     5281 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5341 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     5461 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5581 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     5641 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5701 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5761 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5821 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5881 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5941 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6001 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6061 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6121 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6181 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6241 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     6301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6901 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6961 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8221 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8281 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8341 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8401 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     8461 cactaaaggg aacaaaagct ggagctgcaa gc
//
