LOCUS       sequence-160489-        6681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Tontonoz P, Hu E, Spiegelman BM
  TITLE     Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a 
            lipid-activated transcription factor.
  JOURNAL   Cell. 1994 Dec 30. 79(7):1147-56.
  PUBMED    8001151
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1994 
            Dec 30. 79(7):1147-56."
COMMENT     Sequence Label: pBABE puro PPAR gamma 2
FEATURES             Location/Qualifiers
     source          1..6681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1605..1625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1610..1939
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1790..1925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1949..2548
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1949..1968)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2445..2465
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2769..3238
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3368..3385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3539..4127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3619..3638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4287..5078,5079..5147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4910..4929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5148..5252)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5283..5752
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5817..6016
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6217..6633
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6563..6585
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6601..6617
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gactacaagg acgacgatga caagctcgat ggaggatacc cctacgacgt gcccgactac
       61 gccggaggac tcgagatggg tgaaactctg ggagattctc ctgttgaccc agagcatggt
      121 gccttcgctg atgcactgcc tatgagcact tcacaagaaa ttaccatggt tgacacagag
      181 atgccattct ggcccaccaa cttcggaatc agctctgtgg acctctccgt gatggaagac
      241 cactcgcatt cctttgacat caagcccttt accacagttg atttctccag catttctgct
      301 ccacactatg aagacattcc attcacaaga gctgacccaa tggttgctga ttacaaatat
      361 gacctgaagc tccaagaata ccaaagtgcg atcaaagtag aacctgcatc tccaccttat
      421 tattctgaaa agacccagct ctacaacagg cctcatgaag aaccttctaa ctccctcatg
      481 gccattgagt gccgagtctg tggggataaa gcatcaggct tccactatgg agttcatgct
      541 tgtgaaggat gcaagggttt tttccgaaga accatccgat tgaagcttat ttatgatagg
      601 tgtgatctta actgccggat ccacaaaaaa agtagaaata aatgtcagta ctgtcggttt
      661 cagaagtgcc ttgctgtggg gatgtctcac aatgccatca ggtttgggcg gatgccacag
      721 gccgagaagg agaagctgtt ggcggagatc tccagtgata tcgaccagct gaacccagag
      781 tctgctgatc tgcgagccct ggcaaagcat ttgtatgact catacataaa gtccttcccg
      841 ctgaccaaag ccaaggcgag ggcgatcttg acaggaaaga caacggacaa atcaccattt
      901 gtcatctacg acatgaattc cttaatgatg ggagaagata aaatcaagtt caaacatatc
      961 acccccctgc aggagcagag caaagaggtg gccatccgaa tttttcaagg gtgccagttt
     1021 cgatccgtag aagccgtgca agagatcaca gagtatgcca aaaatatccc tggtttcatt
     1081 aaccttgatt tgaatgacca agtgactctg ctcaagtatg gtgtccatga gatcatctac
     1141 acgatgctgg cctccctgat gaataaagat ggagtcctca tctcagaggg ccaaggattc
     1201 atgaccaggg agttcctcaa aagcctgcgg aagccctttg gtgactttat ggagcctaag
     1261 tttgagtttg ctgtgaagtt caatgcactg gaattagatg acagtgactt ggctatattt
     1321 atagctgtca ttattctcag tggagaccgc ccaggcttgc tgaacgtgaa gcccatcgag
     1381 gacatccaag acaacctgct gcaggccctg gaactgcagc tcaagctgaa tcacccagag
     1441 tcctctcagc tgttcgccaa ggtgctccag aagatgacag acctcaggca gatcgtcaca
     1501 gagcacgtgc agctactgca tgtgatcaag aagacagaga cagacatgag ccttcacccc
     1561 ctgctccagg agatctacaa ggacttgtat taggtcgacc ctgtggaatg tgtgtcagtt
     1621 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1681 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1741 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     1921 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     1981 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2041 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2101 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2161 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2221 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2281 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2341 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2401 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2461 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     2521 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     2581 aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa
     2641 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     2701 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     2761 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     2821 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     2881 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     2941 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     3001 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     3061 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     3121 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     3181 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     3241 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     3301 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4201 tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccgcaaatc aatctaaagt
     4261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgca
     5281 gcctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5341 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     5401 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     5461 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     5521 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     5581 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     5641 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     5701 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     5761 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc
     5821 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
     5881 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     5941 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     6001 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     6061 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     6121 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     6181 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     6241 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     6301 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     6361 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     6421 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     6481 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     6541 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     6601 ctttatccag ccctcactcc ttctctaggc gccggccgga tcccagtgtg gtggtacgta
     6661 ctcgacgctc gatgaaccat g
//
