LOCUS       sequence-160491-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of 
            mCherry and HXGPRT selectable marker cassette for endogenous tagging
            on the gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pmCherry.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..785
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(220..238)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     488..507
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggtaccc tgtacttcca atccaattta attaaaattg
       61 gaagtggagg acggatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      121 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      181 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      241 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      301 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      361 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      421 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      481 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      541 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      601 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      661 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      721 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      781 agtaaggcgc gcccagaagc tgcccgtctc tcgttttcct ctcttttcgg agggatcagg
      841 gagagtgcct cgggtcggag agagctgacg agggggtgcc agagacccct gtgtccttta
      901 tcgaagaaaa gggatgactc ttcatgtggc a
//
