LOCUS       sequence-160499-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing REG1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_REG1A_WT
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(595..694)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(711..730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(712..730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(735..751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(735..751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatggctca gaccagctca tacttcatgc
      121 tgatctcctg cctgatgttt ctgtctcaga gccaaggcca agaggcccag acagagttgc
      181 cccaggcccg gatcagctgc ccagaaggca ccaatgccta tcgctcctac tgctactact
      241 ttaatgaaga ccgtgagacc tgggttgatg cagatctcta ttgccagaac atgaattcgg
      301 gcaacctggt gtctgtgctc acccaggccg agggtgcctt tgtggcctca ctgattaagg
      361 agagtggcac tgatgacttc aatgtctgga ttggcctcca tgaccccaaa aagaaccgcc
      421 gctggcactg gagcagtggg tccctggtct cctacaagtc ctggggcatt ggagccccaa
      481 gcagtgttaa tcctggctac tgtgtgagcc tgacctcaag cacaggattc cagaaatgga
      541 aggatgtgcc ttgtgaagac aagttctcct ttgtctgcaa gttcaaaaac tgattgccaa
      601 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      661 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
      721 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
      781 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
      841 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
      901 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
      961 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgca
//
