LOCUS       sequence-160509-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wtYAP in mammalian cells under the control of a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-wtYAP
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..96
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     494..514
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aattcgactc gtctgagggc ggaggcgaag acgcggaaga ggccgcagag ccggcagcag
       61 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
      121 gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga
      181 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
      241 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
      301 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
      361 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
      421 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
      481 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
      541 acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt gacatggccg
      601 ctctataacc atgtaagaaa gctttcttta tctagcttgg tggcagccaa aacagactcc
      661 atgtcattaa ggatgtcaga actcaaagct tcctgcagac ttggcatcag ctcctctcct
      721 tctatgttca ttccatctcc ttccagtgtt ccaaggtcca catttgtccc aggaatggct
      781 tcaaggtagt ctgggaaacg gttctgctgt gagggcaggg tgctttggtt gatagtatca
      841 cctgtatcca tctcatccac actgttcagg aagtcatctg gggttcgagg gacactgtag
      901 ctgctcatgc ttagtccact gtctgtactc tcatctcgag agtgataggt gccactgtta
      961 aggaaaggat ctgagctatt ggtcgtcatt gttctcaatt cctgagacat cccgggagaa
     1021 gacactggat tttgagtccc accatcctgc tccagtgttg gtaactggct acgcagggct
     1081 aactcctgac attttgga
//
