LOCUS       sequence-160510-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells 
            under the control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-YAPS94A
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..74
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctagctatc aggatcctac gaggcgcgcc agatagaatt cgccaccatg gactacaagg
       61 acgacgacga caaggatccc gggcagcagc cgccgcctca accggccccc cagggccaag
      121 ggcagccgcc ttcgcagccc ccgcaggggc agggcccgcc gtccggaccc gggcaaccgg
      181 cacccgcggc gacccaggcg gcgccgcagg caccccccgc cgggcatcag atcgtgcacg
      241 tccgcgggga ctcggagacc gacctggagg cgctcttcaa cgccgtcatg aaccccaaga
      301 cggccaacgt gccccagacc gtgcccatga ggctccggaa gctgcccgac gcgttcttca
      361 agccgccgga gcccaaatcc cactcccgac aggccagtac tgatgcaggc actgcaggag
      421 ccctgactcc acagcatgtt cgagctcatt cctctccagc ttctctgcag ttgggagctg
      481 tttctcctgg gacactgacc cccactggag tagtctctgg cccagcagct acacccacag
      541 ctcagcatct tcgacagtct tcttttgaga tacctgatga tgtacctctg ccagcaggtt
      601 gggagatggc aaaaaccagc agcgggcaac gttattttct caatcacatc gatcagacaa
      661 caacatggca ggaccccagg aaggccatgc tgtcccagat gaacgtcaca gcccccacca
      721 gtccaccagt gcagcagaat atgatgaact cggcttcagg tcctcttcct gatggatggg
      781 aacaagccat gactcaggat ggagaaattt actatataaa ccataagaac aagaccacct
      841 cttggctaga cccaaggctt gaccctcgtt ttgccatgaa ccagagaatc agtcagagtg
      901 ctccagtgaa acagccacca cccctggctc cccagag
//
