LOCUS       sequence-160516-       14502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses conditional RU486-dependent GAL4 protein under control of 
            the elav promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Osterwalder T, Yoon KS, White BH, Keshishian H
  TITLE     A conditional tissue-specific transgene expression system using 
            inducible GAL4.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12596-601.
  PUBMED    11675495
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2001-10-23"; volume: "98(22)"; pages: 
            "12596-601"
COMMENT     Sequence Label: pP{ELAV-GeneSwitch}
FEATURES             Location/Qualifiers
     source          1..14502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3064..3080
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3065..3081
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8494..8865
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     intron          9131..9196
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9326..9346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9618..9752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9655..9674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9709..9728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9779..9797)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(10257..10328,10723..10996,11071..11725,11787..12102,
                     12323..12454,12525..13139)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(13917..14471,14472..14502)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ccccggatcc agtcgaggga
     3121 tcactgaact aaaaagtaaa gagaggagaa acaggagaag cccttaaagt gcgagttcgc
     3181 tggagtgcct atcgatcctt gagggcagcg ctcgatcctt attactattg gcaggatcat
     3241 tttatacccc ttacataaaa atatcagtat cagtttgttc ccttatcatg cgtgctcttt
     3301 gacatatccg cgggaattta tttctatttt tgcttagtca aactgaagac cattttgtcc
     3361 caacataaaa tatgtaaaat attttttttt atcattttaa attctcaggg attaagtcaa
     3421 tgtttgaaaa cttctagctg acttgcgctc cactaccact cggcatgcgt gcccctgatt
     3481 agtaggccag gtggtgggcc tttggcacga tcgcaaccct aaggactgca accgaatagc
     3541 attgagaagg agaccaggat ttctgcactc aaaggtgaaa ttgtctgagc acggaacacg
     3601 gaaaaacaac aacaaaaaca tggcagacgg aaaacaatgc cgccagcggc aaggagggag
     3661 ggtggaaaca catgccacga ttcagtgtcg aatgtcctgt gatcccacat tcatatattt
     3721 tgttattgtg tgcctgcatc cctcgcctgc ttttaattcc tgcatcgatc cttctttgct
     3781 cctggtctac gaacccgatg tcttatggta attttatgat tattacaatt agattcgggg
     3841 gaaactgcat ctgccgccgt ctgcagatcc gattcatttg gattccgtct gctcaatcca
     3901 gatccatgat gggatcatgg ccatttgggc attggtacat ggaaagttaa aagcgcccta
     3961 ggcgttctaa tccaactcgt ctcaagaaaa acgggtgaat gcatggaacg ctggatctgg
     4021 ttctggaaga tccggctaag tcaacaagaa atatgtaacc tcgccattat agctttactt
     4081 tcgaagcaga aaatgcaatc gagaatatcg aatgcacgct ccaggttcct atggctccta
     4141 gctttcggtg cagcacaaca aatatttggg ttggttcgtt tttccgccac gattttaaaa
     4201 cgctaagggt ggctttttcg gagtccctct catttgagcc gggaaaaaga ccagaccctc
     4261 cctttccctc cccagccact ttccagcttt gttgtaacct cgtgttgtgc ctgcttttta
     4321 atcccgcttt gttgtaacgc aggccatttg ctgtttttgt tttgtttctg gcgtggctaa
     4381 aacggattat gtgacccaac cctttcgatt tcgagtctca ttgtattaca tccttttccc
     4441 aaaaatgact aaaaaagcgt attgcctatt tctggggtgg acagaatgaa acggggtcag
     4501 cacgtggccg caaacttgaa ataccattat ggatccaata ggactgtgaa gagttattag
     4561 tgaaacattt cgaacaaact attcctttaa ttgaaatttg ccaattcccc acttaagcat
     4621 cgcatttgca tatttgtatg ctaaccaaac tctctgcgac gtcgatctgt ctgaatctga
     4681 atccgcatat ctttcgaggg cagcaatagc gccaccccac cccatttccg ttgccactag
     4741 cagcgcacca ccctttgaaa ggacatcaaa aaccaccccc tgcccaaagt tgcgttacaa
     4801 ttcccgaagt gcagtgcagc gtacataaaa catacaatga aatccgataa aattttctat
     4861 gtcctttata tcctcataac atgcccaact ataaaccgag ccaaattctt ctcgtgaaaa
     4921 agttgcacac aacgcgattt cactgtacct tacattgttc tgtaaactgg aacagagcgt
     4981 ccgactggaa gaggcgaagg aaaaaatcag cacagggaac aggaagttca caaggattgg
     5041 gggtgtccga gggtggctac atatatatgg cgatatatgg catacagtat gtgcatacat
     5101 atacatacat atacacatat atgtatgtat gtaccatact atgtggcgtg tctagtggcg
     5161 cgaataacga atacaggagc aatgaagcac acgaacgaag cgaaacgaaa cgaagggaaa
     5221 tattgaaaaa gccagaattg cattacttca aaactatacc ggctcttgga tatgggtcct
     5281 gggtctcggt tccttgcttc tggctttcgc ttcgtagggg gtgtacgagc aatactgcca
     5341 ggagaaacca ccctagcctt tggcgttggt ggttgatagt gcaaaaataa taataacgga
     5401 aacggaacca taaacccaaa acagccctta caacgcgact gatttgtaag gataatggtc
     5461 gcatgcatca taaaacggtt gacgacctcg tcggaaagag ccaacatttt gcaatgattt
     5521 ccaattgctc ctgctatcta ggcccaatgg gccgctaagt acatatgaag catacatagc
     5581 taaatacata tgaagcatac atacatcggg ttaactataa aaccaatgtg ttctagatga
     5641 gtttgttaat tttacaaagt aagttagggc tgctccagtg gttttaagtt gcttaactgg
     5701 acaagagaac ttggctctct cccccatcta tctcttgtct tcgggaaagg taacctccat
     5761 tttgatcggt tgaatttgta tcaaaaagca tgaaaaactt tgaatacagc ataaaccact
     5821 gagagagcag ccgaaagcca ggggtgtatc aaaaggggtt cttagaaata ttccactttg
     5881 caacgattat agactcgttt cttatcagac aaaactttct ccacttctac gtggtattta
     5941 cagaaacaaa acttaaggta aaatgttagg tggctatata ttaaagctat taaaatatat
     6001 taaaggtttc cggaataacc ccctatctcg gcgcgcagat acgcccatgt cgccactctt
     6061 tcgctctcta ccttccactc tcgctccgaa caccattgca accccgagag agctgctcga
     6121 gaggcaacta tgagatatga gaatgagagg ttgctctatg tgaaaatgga aaccgaaacc
     6181 gtcaatgcct caatttcgca tttcattatt tcatttccac gccaaccatc gtgcgccgcg
     6241 gtctgaacgc tcctgccaca gaaaaagaag ataaaagcaa ggaaaattct aatcgaatac
     6301 caaatatcgt gcttgtgtgc tctttccgca attgattttt tttaagtagt gcatgacaat
     6361 aaccgttgag ttgactccaa ccgaagtaac cataactgga agctgacaac agccattggc
     6421 cggaatctca tctcacaccg agcgaaatac ggcgacgcca gcgtttattt attaaaaacg
     6481 attacattac aacaaacacc aaagtcctgg acaaccgaag agagtgtggg agaaaatcga
     6541 agcagagagc aaggaggagc gccgtgagag cgcgcgcgca aggatttgag attgcaaaga
     6601 gaacttagca attagttcgc tttgtttgtc cagccaaaca acaacaacaa ccaaccagct
     6661 ccccaaaacc aactacacac atactcgctc cctaaactaa aacaatagcg gccgcggccg
     6721 ggaacggtgc attggaacgc ggattccccg tgttaattaa caggtaagtg tcttcctcct
     6781 gtttccttcc cctgctattc tgctcaacct tcctatcaga aactgcagta tctgtatttt
     6841 tgctagcagt aatactaacg gttctttttt tctcttcaca ggccaccaag ctaccggtcc
     6901 accatggact cccagcagcc agatctgaag ctactgtctt ctatcgaaca agcatgcgat
     6961 atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg
     7021 aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca
     7081 catctgacag aagtggaatc aaggctagaa agactggaac agctatttct actgattttt
     7141 cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg
     7201 ttagaattcc cgggtgtcga ccagaaaaag ttcaataaag tcagagttgt gagagcactg
     7261 gatgctgttg ctctcccaca gccagtgggc gttccaaatg aaagccaagc cctaagccag
     7321 agattcactt tttcaccagg tcaagacata cagttgattc caccactgat caacctgtta
     7381 atgagcattg aaccagatgt gatctatgca ggacatgaca acacaaaacc tgacacctcc
     7441 agttctttgc tgacaagtct taatcaacta ggcgagaggc aacttctttc agtagtcaag
     7501 tggtctaaat cattgccagg ttttcgaaac ttacatattg atgaccagat aactctcatt
     7561 cagtattctt ggatgagctt aatggtgttt ggtctaggat ggagatccta caaacacgtc
     7621 agtgggcaga tgctgtattt tgcacctgat ctaatactaa atgaacagcg gatgaaagaa
     7681 tcatcattct attcattatg ccttaccatg tggcagatcc cacaggagtt tgtcaagctt
     7741 caagttagcc aagaagagtt cctctgtatg aaagtattgt tacttcttaa tacaattcct
     7801 ttggaagggc tacgaagtca aacccagttt gaggagatga ggtcaagcta cattagagag
     7861 ctcatcaagg caattggttt gaggcaaaaa ggagttgtgt cgagctcaca gcgtttctat
     7921 caacttacaa aacttcttga taacttgcat gatcttgtca aacaacttca tctgtactgc
     7981 ttgaatacat ttatccagtc ccgggcactg agtgttgaat ttccagaaat gatgtctgaa
     8041 gttattgctg ggtcgacgcc catggaattc cagtacctgc cagatacaga cgatcgtcac
     8101 cggattgagg agaaacgtaa aaggacatat gagaccttca agagcatcat gaagaagagt
     8161 cctttcagcg gacccaccga cccccggcct ccacctcgac gcattgctgt gccttcccgc
     8221 agctcagctt ctgtccccaa gccagcaccc cagccctatc cctttacgtc atccctgagc
     8281 accatcaact atgatgagtt tcccaccatg gtgtttcctt ctgggcagat cagccaggcc
     8341 tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc tgcccctgct
     8401 ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct agccccaggc
     8461 cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga aggaacgctg
     8521 tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt gcttggcaac
     8581 agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga gtttcagcag
     8641 ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct gatggagtac
     8701 cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc agctcctgct
     8761 ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga cttctcctcc
     8821 attgcggaca tggacttctc agccctgctg agtcagatca gctcctaagg atcctccgga
     8881 ctagaaaagc cgaattctgc aggaattggg tggcatccct gtgacccctc cccagtgcct
     8941 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     9001 tgcatcattt tgtctgacta ggtgtccttc tataattcga gggtacctct agaggatctt
     9061 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     9121 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     9181 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     9241 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     9301 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     9361 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     9421 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     9481 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     9541 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     9601 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9661 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9721 aactcatcaa tgtatcttat catgtctgga tcggatccac tagaaggcct tagtatgtat
     9781 gtaagttaat aaaacccttt tttggagaat gtagatttaa aaaaacatat ttttttttta
     9841 ttttttactg cactggacat cattgaactt atctgatcag ttttaaattt acttcgatcc
     9901 aagggtattt gaagtaccag gttctttcga ttacctctca ctcaaaatga cattccactc
     9961 aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc
    10021 tgcgtccgct atctctttcg ccaccgtttg tagcgttacc tagcgtcaat gtccgccttc
    10081 agttgcactt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa
    10141 cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt
    10201 ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg
    10261 gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc
    10321 tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc
    10381 gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc
    10441 aatcgcacaa acaaatagtg acacgaaaca gattattctg gtagctgtgc tcgctatata
    10501 agacaatttt taagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactaca
    10561 ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa
    10621 ataaataaaa gtataaacct acttcgtagg atacttcgtt ttgttcgggg ttagatgagc
    10681 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
    10741 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctcctgcca
    10801 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
    10861 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
    10921 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
    10981 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
    11041 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
    11101 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
    11161 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
    11221 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
    11281 gctccctaac ggccagggaa cacctgattt tccaggccat ggtgcggatg ccacgacatc
    11341 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
    11401 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
    11461 ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc gccgcttctg atctgcgatg
    11521 agcccacctc cggactggac tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc
    11581 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
    11641 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
    11701 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagcat
    11761 tacattacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
    11821 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
    11881 cggatcgcca agatatgcga caattttgct attagcaaag tagcccggga tatggagcag
    11941 ttgttggcca ccaaaaattt ggagaagcca ctggagcagc cggagaatgg gtacacctac
    12001 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
    12061 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
    12121 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agttagaatt
    12181 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
    12241 aaaataaata aatttcattt aactcgcgaa catgttgaag atatgaatat taatgagatg
    12301 cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca
    12361 acaactcacg caagtgggcg tgatgaatat caacggagcc atcttcctct tcctgaccaa
    12421 catgaccttt caaaacgtct ttgccacgat aaatgtaagt cttgtttaga atacatttgc
    12481 atattaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc
    12541 tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg
    12601 gcaaaacgat tgccgaatta ccgctttttc tcacagtgcc actggtcttc acggcgattg
    12661 cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg
    12721 tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct
    12781 cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg
    12841 gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat
    12901 ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg
    12961 aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga
    13021 cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg
    13081 tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc
    13141 cgacatatat ccgaaataac tgcttgtttt tttttttacc attattacca tcgtgtttac
    13201 tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat
    13261 gacctataga atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt
    13321 cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac
    13381 ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata
    13441 attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc
    13501 gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt
    13561 cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata
    13621 tttgtatgta caatttattt gctccaatat atttgtatat atttccctca cagctatatt
    13681 tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt
    13741 agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc
    13801 aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat
    13861 aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg
    13921 ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt
    13981 ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt
    14041 tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct
    14101 tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt
    14161 taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac
    14221 aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa
    14281 tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc
    14341 aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg
    14401 cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa
    14461 gcttcggcta tcgacgggac caccttatgt tatttcatca tg
//
