LOCUS       sequence-160525-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP selectable marker for use in two-stage CRISPR-mediated allele 
            replacement strategies in Drosophila. D. americana tan homology arms
            can be replaced with locus of choice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Lamb AM, Walker EA, Wittkopp PJ
  TITLE     Tools and strategies for scarless allele replacement in Drosophila 
            using CRISPR/Cas9.
  JOURNAL   Fly (Austin). 2016 Aug 5:1-12.
  PUBMED    27494619
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin). 2016 Aug 5:1-12."
COMMENT     Sequence Label: pGEM-WingGFP-tan
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcgtt
      301 taaacaggcc tctcgactct agcgcgtacc ctagaggatc agcttggctg cagattgttt
      361 agcttgttca gctgcgcttg tttatttgct tagctttcgc ttagcgacgt gttcactttg
      421 cttgtttgaa ttgaattgtc gctccgtaga cgaagcgcct ctatttatac tccggcgctc
      481 ctcgagctct gtacatgtcg gccggcctag gcgcgccgga aagcaaagcc gaataccgca
      541 tgacattgcc cgacccgtgt ttcatgtttt attttcctta ttccccactt atggcagcct
      601 ggacattatc tccctccctc ccctcccccc tcctacacta atttggaaat ccatcgtgac
      661 tgcaaaacga aaaacccgtt gctcaacttc tctaacataa gcggtacaca gcattttccg
//
