LOCUS       sequence-160526-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP selectable marker for use in two-stage CRISPR-mediated allele 
            replacement strategies in Drosophila. D. americana tan homology arms
            can be replaced with locus of choice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Lamb AM, Walker EA, Wittkopp PJ
  TITLE     Tools and strategies for scarless allele replacement in Drosophila 
            using CRISPR/Cas9.
  JOURNAL   Fly (Austin). 2016 Aug 5:1-12.
  PUBMED    27494619
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin). 2016 Aug 5:1-12."
COMMENT     Sequence Label: pGEM-WingGFP-tan
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    264..385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(301..320)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     355..374
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gagctgtaca agagcaggca cagaaggcat cgccagcgct ctaggagccg caatcgcagc
       61 cgaagtcgca gcagtgaacg aaaacgccgt caacggagcc gaagtcgcag cagtgaacga
      121 agacgctact tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
      181 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      241 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      301 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      361 tgtccaaact catcaatgta tcttactagt ggccacgtgg aggccggccg aattcgaatg
      421 gccatgggac gtcgacctgt ggtaattata accctggcgc ctaaggatcg atacttgggg
      481 ataccctgaa ccctcttctt ccaacaccaa atgcaaatat atatacagac tctatatata
      541 tgcagactct agctcttatt atttacccaa tttgctcaaa aaacaagatt tcgatatcga
      601 tttttatcga ttgcttggaa acggggcaag ttatcgaata tcggaaacaa actcgatctg
      661 cgtagacact agtcaagttc ttcaagtctc tagctcttct gatatccctg ccttcataca
      721 tatggacgga cggacggacg gacatggcta gatcgactcg gctattgatg ctgatcaaga
      781 atatatatac tttatggggt cggaggtgct tcattctgcc tgttacatac atttgcattt
      841 tgcacaaacc cttcttaccc actttcaatg ggttcagagt ataaaatgaa attgtttttg
      901 tgctcatcga attcgaa
//
