LOCUS       sequence-160527-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of 
            tdTomato and HXGPRT selectable marker cassette for endogenous 
            tagging on the gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: ptdTomato.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(215..233)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     483..502
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     677..700
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccctgt acttccaatc caatttaatt
       61 aaaattggaa gtggaggacg gatggtgagc aagggcgagg aggtcatcaa agagttcatg
      121 cgcttcaagg tgcgcatgga gggctccatg aacgaccacg agttcgagat cgagggcgag
      181 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
      241 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
      301 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
      361 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
      421 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
      481 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
      541 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
      601 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
      661 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
      721 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
      781 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag
      841 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcc
//
