LOCUS       sequence-160530-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of 
            tdTomato and HXGPRT selectable marker cassette for endogenous 
            tagging on the gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: ptdTomato.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(823..846)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctgccagaac acttgtcaac cgactgtgtc cacattttta tgcgcactga ctggcatgaa
       61 tggccagagg caggcatcag caagtcacgt aggccaacgc gtgcgcagaa acgctcaagg
      121 ctcgattgtg ggtgggggtt ggtagcattt tatcgaccta aacaaggttt acacttaggt
      181 ggtgcggttt tactgatctg gacggattca gcggtcgcag attatcgatc tgcaaatggt
      241 gtacacttag gtgtcgcggc ttatctagtt aagggagctt cgtggtcgga gcctaacaag
      301 tcaacagaga cgtatcgcca atcgttcgcg gtgaagagtc gaaactgaca gcacatcgta
      361 gggaaactga gagggtgctc ctttctctcc gtcgtttgcg ctgcaccatc ctgcaagtgc
      421 atagaaggaa agttgtctgc tgtcgtgggc agacagcaac agtccagcac tctagcggca
      481 tacagaacga taacgcattc acgagtggat acacgcacat ctgcgtcacc cgcaactcgc
      541 tttcgttctg attgacaaaa agaaaacaag gcgaggtgag actgtgtgaa atgccacatg
      601 aagagtcatc ccttttcttc gataaaggac acaggggtct ctggcacccc ctcgtcagct
      661 ctctccgacc cgaggcactc tccctgatcc ctccgaaaag agaggaaaac gagagacggg
      721 cagcttctgg cgcgccttac ttgtacagct cgtccatgcc gtacaggaac aggtggtggc
      781 ggccctcgga gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      841 ccagcttggt gtccacgtag tagtagccgg gcagttgcac gggcttcttg gccatgtaga
      901 tggtcttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc tggtggatct
      961 cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc ca
//
