LOCUS       sequence-160534-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pLIC-SGC1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(118..151)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        166..611
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat aagctagctt ctcctcctga aagatccata
      121 acttcgtata gcatacatta tacgaagtta tgcggccgcg acgtccacat atacctgccg
      181 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
      241 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
      301 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
      361 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
      421 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
      481 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
      541 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
      601 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
      661 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
      721 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
      781 agcaaaaaaa tgaaaaatat
//
