LOCUS       sequence-160540-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of CFP 
            and HXGPRT selectable marker cassette for endogenous tagging on the 
            gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pCFP.HXGPRT
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(786..807)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcctgcca gaacacttgt caaccgactg tgtccacatt tttatgcgca ctgactggca
       61 tgaatggcca gaggcaggca tcagcaagtc acgtaggcca acgcgtgcgc agaaacgctc
      121 aaggctcgat tgtgggtggg ggttggtagc attttatcga cctaaacaag gtttacactt
      181 aggtggtgcg gttttactga tctggacgga ttcagcggtc gcagattatc gatctgcaaa
      241 tggtgtacac ttaggtgtcg cggcttatct agttaaggga gcttcgtggt cggagcctaa
      301 caagtcaaca gagacgtatc gccaatcgtt cgcggtgaag agtcgaaact gacagcacat
      361 cgtagggaaa ctgagagggt gctcctttct ctccgtcgtt tgcgctgcac catcctgcaa
      421 gtgcatagaa ggaaagttgt ctgctgtcgt gggcagacag caacagtcca gcactctagc
      481 ggcatacaga acgataacgc attcacgagt ggatacacgc acatctgcgt cacccgcaac
      541 tcgctttcgt tctgattgac aaaaagaaaa caaggcgagg tgagactgtg tgaaatgcca
      601 catgaagagt catccctttt cttcgataaa ggacacaggg gtctctggca ccccctcgtc
      661 agctctctcc gacccgaggc actctccctg atccctccga aaagagagga aaacgagaga
      721 cgggcagctt ctggcgcgcc ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      781 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      841 gcggactg
//
