LOCUS       sequence-160544-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of SF-TAP
            and HXGPRT selectable marker cassette for endogenous tagging on the 
            gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pSF-TAP.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             142..165
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             181..204
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccctcactaa agggaacaaa agctggtacc ctgtacttcc aatccaattt aattaaaatt
       61 ggaagtggag gacgggaatt ctggtctcat cctcagtttg aaaaaggtgg tggttctggt
      121 ggtggttccg gtggtggttc ttggtctcat ccgcaattcg aaaagggcgc ctctggtgag
      181 gattacaaag acgacgacga taaataaggc gcgccagaag ctgcccgtct ctcgttttcc
      241 tctcttttcg gagggatcag ggagagtgcc tcgggtcgga gagagctgac gagggggtgc
      301 cagagacccc tgtgtccttt atcgaagaaa agggatgact cttcatgtgg catttcacac
      361 agtctcacct cgccttgttt tctttttgtc aatcagaacg aaagcgagtt gcgggtgacg
      421 cagatgtgcg tgtatccact cgtgaatgcg ttatcgttct gtatgccgct agagtgctgg
      481 actgttgctg tctgcccacg acagcagaca actttccttc tatgcacttg caggatggtg
      541 cagcgcaaac gacggagaga aaggagcacc ctctcagttt ccctacgatg tgctgtcagt
      601 ttcgactctt caccgcgaac gattggcgat acgtctctgt tgacttgtta ggctccgacc
      661 acgaagctcc cttaactaga taagccgcga cacctaagtg tacaccattt gcagatcgat
      721 aatctgcgac cgctgaatcc gtccagatca gtaaaaccgc accacctaag tgtaaacctt
      781 gtttaggtcg ataaaatgct accaaccccc acccacaatc gagccttgag cgtttctgcg
      841 cacgcgttgg cctacgtgac ttgctgatgc ctgcctctgg ccattcatgc cagtcagtgc
      901 gcataaaaat gtggacacag tcggttga
//
