LOCUS       sequence-160553-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For tagging mitochondria with HA epitopes (HA-MITO construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM
  TITLE     Absolute Quantification of Matrix Metabolites Reveals the Dynamics 
            of Mitochondrial Metabolism.
  JOURNAL   Cell. 2016 Aug 25;166(5):1324-1337.e11. doi: 
            10.1016/j.cell.2016.07.040.
  PUBMED    27565352
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1324-1337.e11"
COMMENT     Sequence Label: pMXs-3XHA-EGFP-OMP25
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..67
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             80..106
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(158..160,161..871)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(158..160,161..871)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(200..221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(461..480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     808..829
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggtggtacg ggaattcctg caggcctcga ggccaccatg tatccctatg acgtgcctga
       61 ttacgccggc acaggatcct acccctatga tgtgcctgac tacgctggca gcgccggata
      121 cccttatgat gtgcctgatt atgctggagg gagcggcgtg agcaagggcg aggagctgtt
      181 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      241 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      301 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      361 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      421 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      481 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      541 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      601 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      661 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      721 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      781 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      841 gatcactctc ggcatggacg agctgtacaa gccgcggcat c
//
