LOCUS       sequence-160558-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent signal joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: 289 RFP/CFP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..789
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(242..260)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(292..312)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     510..529
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     704..727
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcccaa gcttgggctg caggtcgact
       61 gcagggtttt tgttccagtc tgtagcactg tggtcgactc tcatcgatac catggcctcc
      121 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
      181 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      241 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
      301 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
      361 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      421 gtggtgaccg tgacccagga ctcctccctg caggacggaa gcttcatcta caaggtgaag
      481 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg
      541 gaggcctcca ccgagcgcct gtacccccgc gacggcgcgc tgaagggcga gatccacaag
      601 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
      661 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
      721 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
      781 ttcctgtagc atcgatacca tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt
      841 caaggtgcgc atggagggct ccgtgaacgg
//
