LOCUS       sequence-160559-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent signal joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: 289 RFP/CFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(111..788)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(173..196)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(371..390)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     588..608
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     640..658
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtgatcc ccggggatcc cgagggtttt
       61 tgtacagcca gacagtggag tactaccact gtgggatcct ctcatcgatg ctacaggaac
      121 aggtggtggc ggccctcggc gcgctcgtac tgctccacga tggtgtagtc ctcgttgtgg
      181 gaggtgatgt ccagcttgga gtccacgtag tagtagccgg gcagctgcac gggcttcttg
      241 gccatgtaga tggacttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc
      301 ttgtggatct cgcccttcag cgcgccgtcg cgggggtaca ggcgctcggt ggaggcctcc
      361 cagcccatag tcttcttctg cattacgggg ccgtcggagg ggaagttcac gccgatgaac
      421 ttcaccttgt agatgaagct tccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      481 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      541 ttgtagtcgg ggatgtcggc ggggtgcttc acgtacacct tggagccgta ctggaactgg
      601 ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg
      661 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      721 ttcacggagc cctccatgcg caccttgaag cgcatgaact ccttgatgac gtcctcggag
      781 gaggccatgg tatcgatg
//
