LOCUS       sequence-160564-       13182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry tagged BUB3 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 13182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUM BUB3
FEATURES             Location/Qualifiers
     source          1..13182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3400..3801
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3815..4519
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(3960..3978)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4228..4247
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5114..5125
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5523..6095
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5690..5707)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5917..5936
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6098..6700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6597..6617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           6707..6828
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           6856..6870
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           6898..7024
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(7119..7137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7157..7745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7210..7230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7628..7639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7952..8185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8286..8303)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8292..8516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8562..8990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8649..8668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8859..8880
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8999..9019)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9004..9333
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9184..9319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9246..9265
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9382..10407
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9382..10407
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10537..10658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10707..10723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10707..10723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10720..10742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10731..10747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10755..10761,10762..10779,10780..10785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10800..10821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10938..10955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11109..11697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11189..11208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11868..12659,12660..12728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12491..12510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12729..12833)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12881..13015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccgcgtgt ggcatctgaa gcaccaccag cgagcgagag ctagagagaa
     2761 ggaaagccac cgacttcacc gcctccgagc tgctccgggt cgcgggtctg cagcgtctcc
     2821 ggccctccgc gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg
     2881 cggggccgcc ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca
     2941 gcgggcgcgc gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc
     3001 tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg
     3061 tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag
     3121 cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt
     3181 cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg
     3241 agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag
     3301 gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac
     3361 tagaagttga ttcgggtgtt tccggaattc ctagctgcag taacgccatt ttgcaaggca
     3421 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     3481 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     3541 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     3601 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     3661 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     3721 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     3781 gtcctccgac agactgagtc gcccgctgga gaccatggtg agcaagggcg aggaggataa
     3841 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3901 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3961 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4021 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4081 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4141 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4201 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4261 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4321 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4381 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4441 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4501 cggcatggac gagctgtaca tgaccggttc taacgagttc aagctgaacc agccacccga
     4561 ggatggcatc tcctccgtga agttcagccc caacacctcc cagttcctgc ttgtctcctc
     4621 ctgggacacg tccgtgcgtc tctacgatgt gccggccaac tccatgcggc tcaagtacca
     4681 gcacaccggc gccgtcctgg actgcgcctt ctacgatcca acgcatgcct ggagtggagg
     4741 actagatcat caattgaaaa tgcatgattt gaacactgat caagaaaatc ttgttgggac
     4801 ccatgatgcc cctatcagat gtgttgaata ctgtccagaa gtgaatgtga tggtcactgg
     4861 aagttgggat cagacagtta aactgtggga tcccagaact ccttgtaatg ctgggacctt
     4921 ctctcagcct gaaaaggtat ataccctctc agtgtctgga gaccggctga ttgtgggaac
     4981 agcaggccgc agagtgttgg tgtgggactt acggaacatg ggttacgtgc agcagcgcag
     5041 ggagtccagc ctgaaatacc agactcgctg catacgagcg tttccaaaca agcagggtta
     5101 tgtattaagc tctattgaag gccgagtggc agttgagtat ttggacccaa gccctgaggt
     5161 acagaagaag aagtatgcct tcaaatgtca cagactaaaa gaaaataata ttgagcagat
     5221 ttacccagtc aatgccattt cttttcacaa tatccacaat acatttgcca caggtggttc
     5281 tgatggcttt gtaaatattt gggatccatt taacaaaaag cgactgtgcc aattccatcg
     5341 gtaccccacg agcatcgcat cacttgcctt cagtaatgat gggactacgc ttgcaatagc
     5401 gtcatcatat atgtatgaaa tggatgacac agaacatcct gaagatggta tcttcattcg
     5461 ccaagtgaca gatgcagaaa caaaacccaa gtcaccatgt acttgagcgg ccgcaaattc
     5521 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5581 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5641 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5701 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5761 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5821 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5881 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5941 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6001 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6061 ggggacgtgg ttttcctttg aaaaacacga taataccatg gccaccgagt acaagcccac
     6121 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     6181 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     6241 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6301 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6361 gttcgccgag atcggctcgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6421 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6481 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6541 agtggaggcg gccgagcgcg ctggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6601 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6661 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtttgtttga atgaggcttc
     6721 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
     6781 ggttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gatctcgctt
     6841 gggcgagagt aagtagtgaa gccacagatg tacttactct cgcccaagcg agagtgccta
     6901 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
     6961 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
     7021 cttttttcaa ttggaagact aatgcggccg gccattactc cgtctcgtgt cttgttgcat
     7081 atgtctgctg gtttgtttga tgttgtttgc gggcgggccc tatagtgagt cgtattacct
     7141 aggacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7201 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7261 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7321 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7381 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7441 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7501 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7561 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7621 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7681 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7741 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     7801 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7861 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7921 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     7981 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8041 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8101 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8161 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8221 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgttt aaacccgctg
     8281 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8341 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8401 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     8461 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     8521 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     8581 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8641 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8701 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8761 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8821 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8881 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8941 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     9001 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9061 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9121 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9181 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9241 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     9301 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     9361 ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     9421 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     9481 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     9541 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     9601 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     9661 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     9721 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     9781 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     9841 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     9901 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     9961 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10021 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    10081 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    10141 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    10201 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    10261 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    10321 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    10381 agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc
    10441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    10501 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    10561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    10681 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12841 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    12901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    12961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    13021 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    13081 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    13141 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
