LOCUS       sequence-160565-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent coding joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: 290 RFP/CFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..772
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(225..243)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(275..295)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     493..512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     687..710
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagctcg accacagtgc tacagactgg
       61 aacaaaaacc ctgcagtcga ctctcatcga taccatggcc tcctccgagg acgtcatcaa
      121 ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat
      181 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      241 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
      301 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
      361 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      421 ggactcctcc ctgcaggacg gaagcttcat ctacaaggtg aagttcatcg gcgtgaactt
      481 cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct ccaccgagcg
      541 cctgtacccc cgcgacggcg cgctgaaggg cgagatccac aaggccctga agctgaagga
      601 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct
      661 gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac
      721 catcgtggag cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt agcatcgata
      781 ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
      841 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgaggg
//
