LOCUS       sequence-160602-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing GZMA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GZMA_WT
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(883..982)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(999..1018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1000..1018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgaggaa ctccaataga tttctggcat
      121 cctctctctc agttgtcgtt tctctcctgc taattcctga agatgtctgt gaaaaaatta
      181 ttggaggaaa tgaagtaact cctcattcaa gaccctacat ggtcctactt agtcttgaca
      241 gaaaaaccat ctgtgctggg gctttgattg caaaagactg ggtgttgact gcagctcact
      301 gtaacttgaa caaaaggtcc caggtcattc ttggggctca ctcaataacc agggaagagc
      361 caacaaaaca gataatgctt gttaagaaag agtttcccta tccatgctat gacccagcca
      421 cacgcgaagg tgaccttaaa cttttacagc tgacggaaaa agcaaaaatt aacaaatatg
      481 tgactatcct tcatctacct aaaaaggggg atgatgtgaa accaggaacc atgtgccaag
      541 ttgcagggtg gggcaggact cacaatagtg catcttggtc cgatactctg agagaagtca
      601 atatcaccat catagacaga aaagtctgca atgatcgaaa tcactataat tttaaccctg
      661 tgattggaat gaatatggtt tgtgctggaa gcctccgagg tggaagagac tcgtgcaatg
      721 gagattctgg aagccctttg ttgtgcgagg gtgttttccg aggggtcact tcctttggcc
      781 ttgaaaataa atgcggagac cctcgtgggc ctggtgtcta tattcttctc tcaaagaaac
      841 acctcaactg gataattatg actatcaagg gagcagtttg attgccaact ttcttgtaca
      901 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      961 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     1021 tggtc
//
