LOCUS       sequence-160609-        6594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse neurofilament protein M cDNA mammalian expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Yan Y, Jensen K, Brown A
  TITLE     The polypeptide composition of moving and stationary neurofilaments 
            in cultured sympathetic neurons.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309.
  PUBMED    17285620
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton."; date: "2007-04"; volume: "64(4)"; pages: "299-309"
COMMENT     Sequence Label: pmNFM
FEATURES             Location/Qualifiers
     source          1..6594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3382..3503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3419..3438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3473..3492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3510..3965)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3647..3668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3859..3878
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3992..4096
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4098..4455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4098..4289
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4121..4141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4306..4441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4490..5284
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4490..5284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4544..4563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5154..5173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5472..5491)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5516..5563
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5892..6480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6381..6400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccgggatccg cctccaagat gagctacacg ctggactcgc tgggcaaccc gtccgcctac
      661 cggcgggtca ccgagacccg ctccagcttc agccgcgtga gcggttcccc gtccagcggc
      721 ttccgctcgc agtcctggtc ccgcggctcg cccagcaccg tgtcctcctc ctacaagcgc
      781 agcgcgctcg ccccgcgtct cgcctacagc tcggctatgc tcagctcggc cgagagcagc
      841 ctcgacttca gccagtcctc gtcgctgctc aacggcggct ccggcggcga ctacaaactg
      901 tcccgctcta acgagaaaga gcagctgcag gggctgaacg accgcttcgc cggctacatc
      961 gagaaagtgc actacttgga acaacagaac aaggagatcg aagcagagat ccaggcactg
     1021 cggcagaagc aggcctcgca cgcccagctg ggtgatgctt acgaccagga gatccgagag
     1081 ctgcgcgcca ccctcgagat ggtgaaccac gagaaggctc aagtgcagct ggactccgat
     1141 cacttggagg aagacatcca ccggctcaag gagcgcttcg aggaggaggc gcggctgcgg
     1201 gacgacaccg aggctgccat tcgcgcgctg cgcaaagaca tcgaagagtc gtcgatggtt
     1261 aaggtggagc tggacaagaa ggtgcagtcg ctgcaggatg aggtggcttt cctgcggagc
     1321 aatcacgaag aggaggtggc cgacctgctg gctcagatcc aggcgtcgca catcacggta
     1381 gagcgcaaag attacctgaa gacagacatc tccacggcgc tgaaggagat ccgctcccag
     1441 ctcgagtgtc actcagacca gaacatgcac caggccgaag agtggttcaa atgccgctac
     1501 gccaagctca ccgaggcggc cgagcagaac aaggaggcca ttcgctctgc caaggaagag
     1561 atcgccgagt accggcgcca gctgcagtcc aagagcatcg agctcgagtc ggtgcgaggc
     1621 actaaggagt ccctggagcg gcagctcagc gacatcgagg agcgccacaa ccacgacctc
     1681 agcagctacc aggacaccat ccagcagttg gaaaatgaac ttcggggaac caagtgggaa
     1741 atggctcgtc atttgcgaga ataccaggat ctccttaacg tcaagatggc cctggacatc
     1801 gagatcgccg cgtacaggaa actcctagag ggggaagaga ccagatttag cacattttca
     1861 ggaagcatca ccgggcctct gtacacacac cgacagccct cagtcacaat atccagtaag
     1921 attcagaaga ccaaagtcga ggcccccaag ctcaaggtcc aacacaaatt tgtggaggag
     1981 atcatcgaag aaactaaagt ggaagatgag aagtcagaaa tggaagaaac cctcacagcc
     2041 atcgcagagg agttggcagc ctccgccaaa gaggagaagg aagaggccga agaaaaggag
     2101 gaggaaccag aagccgaaaa gtctcccgtg aagtctcctg aggctaagga agaggaggag
     2161 gaaggggaaa aggaggaaga agaggaaggc caggaggaag aagaggagga agatgaaggt
     2221 gtcaagtcag accaggcaga agagggggga tctgagaagg aaggctccag tgaaaaagat
     2281 gaaggtgagc aggaagaaga agaaggagaa accgaggcag aaggtgaagg agaggaagca
     2341 gaagctaagg aggaaaagaa aattgaggga aaggttgagg aagtggctgt caaggaggaa
     2401 atcaaggtcg agaagcctga gaaagccaaa tcccctatgc ccaaatcacc cgtggaagaa
     2461 gtaaagccaa aaccagaggc caaggccggg aagggtgagc acaaggagga agagaaagtt
     2521 gaggaagaga agaaggaagt caccaaagaa tcacccaagg aagagaaggt ggagaaaaag
     2581 gaggagaagc caaaagatgt tgcagataaa aagaaggccg agtccccggt gaaagagaag
     2641 gctgtggagg aggtgatcac catcagcaag tcggtaaagg tgagcctaga gaaagacacc
     2701 aaagaggaga agctgcagcc gcaggagaag gtgaaggaga aggcagagga ggaggggggc
     2761 agtgaggagg aagggagtga ccgtagcccg caggagtcca agaaggaaga catagctatc
     2821 aatggggagg tggaaggaaa agaggaggag gagcaggaaa ctcaggagaa gggcagtggg
     2881 cgggaggagg agaaaggggt ggtcactaat ggcttagatg tgagccctgc agaggagaag
     2941 aaaggagagg atagcagtga tgataaagtg gtggtcacca agaaggtaga aaagatcacc
     3001 agcgaggggg gcgatggtgc taccaaatac atcaccaaat ctgtaaccgt cactcaaaag
     3061 gttgaagagc atgaggagac ctttgaggag aagctggtct caactaaaaa ggtagaaaag
     3121 gtcacttcac acgccatagt caaggaagtc acccagggtg actaagatcg gagtccgttg
     3181 caaaaggtta agccatacga caatttcaaa atgcatgtga ttgacagctt caaaacagaa
     3241 tgggttctcc caggatccac cggatctaga taactgatca taatcagcca taccacattt
     3301 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3361 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3421 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3481 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     3541 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3601 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3661 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3721 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3781 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3841 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3901 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3961 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4081 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4441 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4501 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4561 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4621 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4681 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4741 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4801 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4861 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4921 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4981 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     5041 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5101 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5161 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5221 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5281 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5341 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5401 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5461 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     5521 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     5581 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     5641 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     5701 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6001 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6541 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
