LOCUS       sequence-160634-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing U2AF1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_U2AF1_WT
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(934..953)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(935..953)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(958..974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(958..974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatggcgga gtatctggcc tccatcttcg
      121 gcaccgagaa agacaaagtc aactgttcat tttatttcaa aattggagca tgtcgtcatg
      181 gagacaggtg ctctcggttg cacaataaac cgacgtttag ccagaccatt gccctcttga
      241 acatttaccg taaccctcaa aactcttccc agtctgctga cggtttgcgc tgtgccgtga
      301 gcgatgtgga gatgcaggaa cactatgatg agttttttga ggaggttttt acagaaatgg
      361 aggagaagta tggggaagta gaggagatga acgtctgtga caacctggga gaccacctgg
      421 tggggaacgt gtacgtcaag tttcgccgtg aggaagatgc ggaaaaggct gtgattgact
      481 tgaataaccg ttggtttaat ggacagccga tccacgccga gctgtcaccc gtgacggact
      541 tcagagaagc ctgctgccgt cagtatgaga tgggagaatg cacacgaggc ggcttctgca
      601 acttcatgca tttgaagccc atttccagag agctgcggcg ggagctgtat ggccgccgtc
      661 gcaagaagca tagatcaaga tcccgatccc gggagcgtcg ttctcggtct agagaccgtg
      721 gtcgtggcgg tggcggtggc ggtggtggag gtggcggcgg acgggagcgt gacaggaggc
      781 ggtcgagaga tcgtgaaaga tctgggcgat tctgattgcc aaactttctt gtacaaagtt
      841 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      901 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
      961 atagctgttt cctggcagct ctggccc
//
