LOCUS       sequence-160762-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human mutant PC5A 
            (T288P) with C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 PCSK5 (T288P)-V5 blast
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca ttactacgta
       61 gaatcgagac cgaggagagg gttagggata ggcttaccaa ccactttgta caagaaagtt
      121 gggcagcctt gaaatgtaca tgttttgcag caaagttgtt gaagaacctt ccgttggcac
      181 agattgttct ttttcacaag catacagaag cctccttccg cccaggactc ttccgttgca
      241 tccttgcaaa tggctcccat ttgacacatt cctaagtcta agagataccc actagggcag
      301 cttgtacagt tcttgaatcc tgggccattg cacgtcaaac aactgatatc acattttttg
      361 caggatttgt atccattctc tgaagagtgg tcaaagtaat agctgatact acagctctgc
      421 acgcatctcc catcctccat gaagtagccc tctgtgcagt taatgcaccc atcagctcct
      481 gccccagcac aagtggcgca gaagcggtgg cagggctggc agtcctggcc gttgaaatac
      541 cgtccatctt cacaggagac agagcaccgg gatccctgca ggcttaaccc atccctacat
      601 tctgtacagt tatggaattc agtacatgtc ttgcagtttt cactgcattt ccggcaaaga
      661 tttttcttgg tatcctgata tgacccatca gggcagtgag taacacagct gttggtttct
      721 tcattcagaa agtatccata tttgcaggac atgcattggt caccatggct cccaa
//
