LOCUS       sequence-160783-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  conditional expression of an shRNA targeting murine Raptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pSico-shRaptor
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(225..258)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     325..341
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     326..342
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..490)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(888..899)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      181 cgggatcact ctcggcatgg acgagctgta caagtagcgg ccgcataact tcgtatagta
      241 taaattatac gaagttataa gccttgtttg cccgagtctg tgaatgtaat ctcgagatta
      301 cattcacaga ctcgggcttt tttctcgagg tcgacggtat cgataagctc gcttcacgag
      361 atcatgttta agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc
      421 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      481 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
      541 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
      601 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
      661 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
      721 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
      781 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
      841 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
      901 cagcggacct tccttcccgc ggcctgctgc cggctctgc
//
