LOCUS       sequence-160793-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing RHOA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RHOA_WT
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             93..674
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     protein_bind    complement(676..775)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(816..832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(816..832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatggctgc catccggaag aaactggtga
      121 ttgttggtga tggagcctgt ggaaagacat gcttgctcat agtcttcagc aaggaccagt
      181 tcccagaggt gtatgtgccc acagtgtttg agaactatgt ggcagatatc gaggtggatg
      241 gaaagcaggt agagttggct ttgtgggaca cagctgggca ggaagattat gatcgcctga
      301 ggcccctctc ctacccagat accgatgtta tactgatgtg tttttccatc gacagccctg
      361 atagtttaga aaacatccca gaaaagtgga ccccagaagt caagcatttc tgtcccaacg
      421 tgcccatcat cctggttggg aataagaagg atcttcggaa tgatgagcac acaaggcggg
      481 agctagccaa gatgaagcag gagccggtga aacctgaaga aggcagagat atggcaaaca
      541 ggattggcgc ttttgggtac atggagtgtt cagcaaagac caaagatgga gtgagagagg
      601 tttttgaaat ggctacgaga gctgctctgc aagctagacg tgggaagaaa aaatctgggt
      661 gccttgtctt gtgattgcca actttcttgt acaaagttgt cattataaga aagcattgct
      721 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      781 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
      841 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
      901 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
      961 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1021 gtaagctgta atgcaagt
//
