LOCUS       sequence-160808-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing TNF , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TNF_WT
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(796..895)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(912..931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(913..931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(936..952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(936..952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgagcac tgaaagcatg atccgggacg
      121 tggagctggc cgaggaggcg ctccccaaga agacaggggg gccccagggc tccaggcggt
      181 gcttgttcct cagcctcttc tccttcctga tcgtggcagg cgccaccacg ctcttctgcc
      241 tgctgcactt tggagtgatc ggcccccaga gggaagagtt ccccagggac ctctctctaa
      301 tcagccctct ggcccaggca gtcagatcat cttctcgaac cccgagtgac aagcctgtag
      361 cccatgttgt agcaaaccct caagctgagg ggcagctcca gtggctgaac cgccgggcca
      421 atgccctcct ggccaatggc gtggagctga gagataacca gctggtggtg ccatcagagg
      481 gcctgtacct catctactcc caggtcctct tcaagggcca aggctgcccc tccacccatg
      541 tgctcctcac ccacaccatc agccgcatcg ccgtctccta ccagaccaag gtcaacctcc
      601 tctctgccat caagagcccc tgccagaggg agaccccaga gggggctgag gccaagccct
      661 ggtatgagcc catctatctg ggaggggtct tccagctgga gaagggtgac cgactcagcg
      721 ctgagatcaa tcggcccgac tatctcgact ttgccgagtc tgggcaggtc tactttggga
      781 tcattgccct gtgattgcca actttcttgt acaaagttgt cattataaga aagcattgct
      841 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      901 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
      961 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaa
//
