LOCUS       sequence-160821-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLJM5-C234S PHGDH
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gnggnatggg cgtagcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
       61 cgtcagatcc gctagcccag caatggcttt tgcaaatctg cggaaagtgc tcatcagtga
      121 cagcctggac ccttgctgcc ggaagatctt gcaagatgga gggctgcagg tggtggaaaa
      181 gcagaacctt agcaaagagg agctgatagc ggagctgcag gactgtgaag gccttattgt
      241 tcgctctgcc accaaggtga ccgctgatgt catcaacgca gctgagaaac tccaggtggt
      301 gggcagggct ggcacaggtg tggacaatgt ggatctggag gccgcaacaa ggaagggcat
      361 cttggttatg aacaccccca atgggaacag cctcagtgcc gcagaactca cttgtggaat
      421 gatcatgtgc ctggccaggc agattcccca ggcgacggct tcgatgaagg acggcaaatg
      481 ggagcggaag aagttcatgg gaacagagct gaatggaaag accctgggaa ttcttggcct
      541 gggcaggatt gggagagagg tagctacccg gatgcagtcc tttgggatga agactatagg
      601 gtatgacccc atcatttccc cagaggtctc ggcctccttt ggtgttcagc agctgcccct
      661 ggaggagatc tggcctctct gtgatttcat cactgtgcac actcctctcc tgccctccac
      721 gacaggcttg ctgaatgaca acacctttgc ccagtgcaag aagggggtgc gtgtggtgaa
      781 ctctgcccgt ggagggatcg tggacgatgg cgccctgctc cgggccctgc agtctggcca
      841 gtgtgccggg gctgcactgt acgtgtttac ggaagagccg ccacgggacc gggtcttggt
      901 ggaccatgag aatgtcatca gctgtcctcc acctggtgcc agcaccaatg aggctcagan
      961 cgctgtgggg gagaaattgc tgtcaggtcg tggacatgtg agggaatctc tcacggggtg
     1021 tgatgccagg cctacagtgc tctctcacac acagcttgna ttgnnntgna gagctctggg
     1081 nacctggatg cgancctggn tggtccnang gnaccatcca ggtggatanc tcangganca
     1141 ct
//
