LOCUS       sequence-160822-        1187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLJM5-C234S PHGDH
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tncttctgcc tgtaccccca atcccccctt ttcttttaaa ttgtggatga atactgccat
       61 ttgtctcgag gtcgagaatt catggtacct cattagaagt ggaactggaa ggcttcagtc
      121 acatgctgct tccacgcttc caggctgggc agcaaggagg agatgcccat gacgtgccag
      181 gtctccccat ctgacaccag tgaagtctgg taggacagca gccgcacgcc tgcctctgcc
      241 aggaggccaa tcatggtagg cagcattgca gggtcagagg tctgagtccg gaataggagc
      301 aggggcaggt ccctgcggag aggcacttct ggcctgaaga cagctccatt gagcccctgc
      361 agtacaggtg tagtgccttg gaccaagccc acagcctggt aaggggcgcc tgccagggcc
      421 acggccagga ggcattcccc gaagccttgc tcccctggtg cagcagggct gtgggaggtg
      481 gtgacattga ggccagcctc tttcaccagc agcttagcgt tcaccaagtt cacatccgcc
      541 tgcttggaag cctctttcag gaggccgaca atgactgcgg ggcttaggca gttcccagca
      601 ttcttcaggg atgttccctg tgttatcacc tggatggtcc ctttggggga cccagcccag
      661 gctcgcatca gtgtccccag agcttctgcc agaccaatcc aaggcttggt gtgtggagag
      721 aaggcactgg taagggcctg ggcattcaca acccccgtga gagatttccc cttcaccatg
      781 tccacgaact gaacagcaat ttcctcccca cagcggctct gagcctcctt ggtgctggca
      841 cccaggtggg gacagctgat gacattctca tggttccacc aaagcccggg tcccgtggcg
      901 gctcttcccg taaacacgtc cagtgcagcc ccggcacact ggtcagactg caggtctcgg
      961 agcaggcgcc ttcgtccacg atccctccca cgggcagagt caccacacgc cacctccctc
     1021 tgcactgggc aagngtgtca tcagccagct gtctgaggca ggaaggatgt gcacagtgat
     1081 gaatcccgaa ggtcagatct ctcaanggnc agctgctgaa cncangaagn cganacnctt
     1141 ggaatgatng nnncnnatag ctcatccaag gactgcatcc gggtagc
//
