LOCUS       sequence-160824-        9447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLJM5-mSHMT1
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1858..1975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2467..2486
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             3955..4188
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4260..4381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4297..4316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4351..4370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4576..4595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4577..4595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4605..4622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4605..4621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4614..4636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4763..5218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4850..4869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5060..5081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5244..5348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5349..5417,5418..6209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5567..5586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6380..6968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6869..6888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7122..7139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7256..7277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7292..7297,7298..7315,7316..7322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7330..7346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7335..7357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7354..7370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7354..7370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7389..7409
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7391..7409
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7437..7663
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7664..7844
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7891..8016
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8509..8742
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8927..8971
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9269..9289
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcagtgcaat ggcagacagg gatgccaccc tgtgggcttc tcatgagaag atgttgtcgc
      421 agcccctcaa ggacagtgat gccgaggttt acagcatcat taaaaaggag agcaaccggc
      481 agagggtcgg attagagctg attgcctcgg agaattttgc cagccgagct gtgttggagg
      541 ccctgggatc ttgcttaaat aacaaatact ctgagggata tccaggccaa aggtattatg
      601 gcgggaccga gttcatcgat gagctggaga tgctgtgtca gaagcgtgca ttacaggcct
      661 accatctgga tcctcagtgc tggggtgtca atgtccagcc ttattcaggc tcccctgcaa
      721 actttgctgt gtatactgcc ctggtagagc ctcatgggcg aatcatgggc ttggacctgc
      781 cagacggtgg ccatctgacc catggcttca tgacagacaa gaagaaaatc tctgccacat
      841 ctatcttctt tgaatccatg ccttataagg tgtacccaga gactgggtac atcaactatg
      901 accagctgga ggagaatgcc agcctcttcc acccgaagct gatcatcgca gggaccagct
      961 gctactcccg gaacctggac tacgcacggc tgcggaagat tgcggatgat aatggggcgt
     1021 atctcatggc agacatggca catatcagtg ggctcgtggc agctggggtg gtgccctccc
     1081 cctttgaaca ctgccatgta gtgaccacta caacccacaa gaccctgaga ggctgccgtg
     1141 ctggtatgat attctacaga aagggtgtgc gcagtgtgga tcccaagact ggcaaagaga
     1201 cttactatga actggagtcc ctcatcaact ctgccgtgtt cccaggcctt caggggggtc
     1261 ctcacaacca tgccattgct ggtgttgctg tggccctgaa gcaagccatg actacagaat
     1321 tcaaaatcta ccagctccag gtgttggcca actgcagggc tctgtctgat gcactcacag
     1381 agctaggcta caaaatagtc acaggtggct ctgataatca cttgatccta atggatctgc
     1441 gttctaaggg tactgatggt ggaagggctg agaaggtact agaagcctgt tctatcgcct
     1501 gcaacaagaa tacctgccca ggtgacaaga gcgcgttacg gcccagtgga ctgcgactgg
     1561 ggactccagc attgacatcc cgaggacttt tggaagaaga cttccaaaaa gtagcacact
     1621 ttatccacag ggggatagag ttgacactgc agattcagag ccacatggcc acgaaggcca
     1681 cactgaaaga gttcaaagag aagctggcag gagatgagaa gatccagagt gctgtggcaa
     1741 ctctccggga agaagtggag aactttgctt ccaacttctc actacctggc cttcctgact
     1801 tctgatgcgg taccatgaat tctcgacctc gagacaaatg gcagtattca tccacaattt
     1861 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1921 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1981 ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc
     2041 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     2101 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     2161 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     2221 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     2281 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     2341 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     2401 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     2461 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     2521 ctccgggcct ttcgacctgc atcccgccac catgaaaaag cctgaactca ccgcgacgtc
     2581 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     2641 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     2701 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     2761 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     2821 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     2881 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     2941 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3001 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3061 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3121 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     3181 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     3241 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     3301 gcggaggcat ccggagcttg caggatcgcc gcggctccgg ggcgtatatg ctccgcattg
     3361 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     3421 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     3481 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     3541 accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgaacaaga
     3601 gctgatttcg agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga
     3661 gaacggcctt acgcttggtg gcacagttct cgtccacagt tcgctaagct cgctcggctg
     3721 ggtcgcggga gggccggtcg cagtgattca ggcccttctg gattgtgttg gtccccaggg
     3781 cacgattgtc atgcccacgc actcgggtga tctgactgat cccgcagatt ggagatcgcc
     3841 gcccgtgcct gccgattggg tgcagatccg tcgacctgca gccaagctct ttaagaccaa
     3901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3961 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4201 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4381 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4501 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4561 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4741 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5101 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7261 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7321 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7381 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7441 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7501 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7561 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7621 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7681 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7741 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7801 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7861 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7921 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7981 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8041 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8101 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8161 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8221 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8281 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8341 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8401 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8461 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8521 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8581 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8641 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8701 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8761 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8821 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8881 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8941 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9001 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9061 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9121 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     9181 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     9241 ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
     9301 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     9361 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     9421 aaaattatgt tttaaaatgg actatca
//
