LOCUS       sequence-160827-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLJM5-mSHMT2
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1936..2053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2545..2564
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             4033..4266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4338..4459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4375..4394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4429..4448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4499..4634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4654..4673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4655..4673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4683..4700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4683..4699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4692..4714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4841..5296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4928..4947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5138..5159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5322..5426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5427..5495,5496..6287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5645..5664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6458..7046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6947..6966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7200..7217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7334..7355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7370..7375,7376..7393,7394..7400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7408..7424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7413..7435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7432..7448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7432..7448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7467..7487
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7469..7487
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7515..7741
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7742..7922
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7969..8094
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8587..8820
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9005..9049
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9347..9367
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgtagcgat ggtatccttc tctttgcttc ggaccactcg gcctctgcag agatgtggtc
      421 agctggtctg catggctgcc cgggcccagc acagcaaggt ggcccagacg caggctgggg
      481 aagcagctgg aggttggacg ggccaggaga gtttatcaga cagtgaccct gagatgtggg
      541 agcttctgca gagggagaag gacagacagt gtcgcggcct ggagctcatc gcctcagaga
      601 acttctgcag ccgagctgcg ctggaggccc tggggtcctg tctcaacaac aagtactcgg
      661 agggttaccc tggcaagaga tactacggag gagcggaagt ggtggacgag atcgagctgc
      721 tctgccagcg ccgggccttg gaagcctttg acctggatcc ggcacagtgg ggagtcaatg
      781 tgcagccata ctcagggtcc ccagccaatc tggctgccta tacggccctt ctgcagcctc
      841 atgatcgaat catggggttg gacctgcccg atgggggcca tctcacccat ggctacatgt
      901 ctgatgtcaa gcggatctcc gccacatcca ttttcttcga gtctatgccc tataagctca
      961 atccccaaac tggcctcatc gactacgacc agctggcgct gaccgctcgg cttttccgac
     1021 cgcggctcat catagctggc acgagtgcct atgcccgcct cattgactat gcacgcatga
     1081 gagaggtctg tgatgaggtc agggcacacc tgctggcaga catggcccac atcagtggcc
     1141 tggtggctgc caaggtgatc ccctcccctt tcaagtacgc ggatgttgtt accaccacca
     1201 ctcacaagac actgcgaggg gccaggtcag ggctcatctt ctaccggaag ggagtacgaa
     1261 ccgtagaccc caagactggc aaagagatcc cttatacctt tgaggaccga atcaacttcg
     1321 ctgtgttccc atccctacag ggtggccccc acaaccacgc cattgctgca gtagccgtgg
     1381 ctctcaagca ggcctgcacc cctatgttcc gcgagtactc cttacaagtg ctgaggaacg
     1441 cccaggccat ggctgatgcc ctgctcaagc gaggatactc gctggtgtct ggtggcactg
     1501 acacccacct ggtgctggtg gacctgcggc ccaagggcct ggatggagcc cgagccgaac
     1561 gcgtgttgga actcgtctcc atcacagcca acaagaacac ctgtcctgga gaccggagcg
     1621 ccattactcc ggggggcttg aggcttgggg cccccgcgtt gacctctcgc cagttccgtg
     1681 aggacgactt ccgtagagtc gtcgatttta tcgatgaggg agtcaacatt ggcttggagg
     1741 tgaagcgcaa gactgccaag ctccaggatt tcaaatcctt cctgctcaaa gacccagaga
     1801 caagccagcg tttggccaac ctccggcaac aggtggaaca gtttgccagg ggcttcccca
     1861 tgcctggatt tgatgaacgt tgatgcggta ccatgaattc tcgacctcga gacaaatggc
     1921 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     1981 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2041 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     2101 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     2161 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     2221 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     2281 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     2341 cggaagccgc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     2401 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     2461 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     2521 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     2581 tgttctcctc ttcctcatct ccgggccttt cgacctgcat cccgccacca tgaaaaagcc
     2641 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     2701 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     2761 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     2821 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     2881 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     2941 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     3001 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     3061 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     3121 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     3181 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     3241 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     3301 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     3361 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggg
     3421 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3481 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3541 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3601 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     3661 gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct
     3721 agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc
     3781 gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga
     3841 ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc
     3901 cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatccgtc gacctgcagc
     3961 caagctcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4081 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4141 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4201 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4261 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4321 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4381 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4441 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4501 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4561 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4621 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4681 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4741 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4801 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5281 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5341 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5401 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5521 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5581 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5641 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5701 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5821 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5941 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6001 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6301 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7141 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7201 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7261 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7321 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7381 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7441 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7501 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7561 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7621 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7681 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7741 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7801 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7861 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7921 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7981 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8041 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8101 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8161 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8221 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8281 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8341 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8401 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8461 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8521 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8581 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8641 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8701 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8761 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8821 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8881 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8941 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9001 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9061 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9121 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9181 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     9241 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     9301 acggtatcga tcacgagact agcctcgagc ggccgccccc ttcaccgagg gcctatttcc
     9361 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     9421 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     9481 tgggtagttt gcagttttaa aattatgttt taaaatggac tatca
//
