LOCUS       sequence-160839-        1334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PHGDH
FEATURES             Location/Qualifiers
     source          1..1334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        91..109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    110..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             186..203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcccatggcc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
       61 tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag
      121 cggataacaa ttcccctcta gaaataattt tgtttaaact ttaagaagga gatatacata
      181 tgaaacatca tcatcatcat caccagatgg cttttgcaaa tctgcggaaa gtgctcatca
      241 gtgacagcct ggacccttgc tgccggaaga tcttgcaaga tggagggctg caggtggtgg
      301 aaaagcagaa ccttagcaaa gaggagctga tagcggagct gcaggactgt gaaggcctta
      361 ttgttcgctc tgccaccaag gtgaccgctg atgtcatcaa cgcagctgag aaactccagg
      421 tggtgggcag ggctggcaca ggtgtggaca atgtggatct ggaggccgca acaaggaagg
      481 gcatcttggt tatgaacacc cccaatggga acagcctcag tgccgcagaa ctcacttgtg
      541 gaatgatcat gtgcctggcc aggcagattc cccaggcgac ggcttcgatg aaggacggca
      601 aatgggagcg gaagaagttc atgggaacag agctgaatgg aaagaccctg ggaattcttg
      661 gcctgggcag gattgggaga gaggtagcta cccggatgca gtcctttggg atgaagacta
      721 tagggtatga ccccatcatt tccccagagg tctcggcctc ctttggtgtt cagcagctgc
      781 ccctggagga gatctggcct ctctgtgatt tcatcactgt gcacactcct ctcctgccct
      841 ccacgacagg cttgctgaat gacaacacct ttgcccagtg caagaagggg gtgcgtgtgg
      901 tgaactgtgc ccgtggaggg atcgtgcgac gaaagcgccc tgctccgggc cctgcagtct
      961 ggccagtgtg ccggggctgc acctggacgt gtttacggaa gaagccgcca ccgggaccgg
     1021 gccttggtgg accatgagaa ttgtcatcag cttgttcccc acctggggtg ccacaccaaa
     1081 ggaggcttca aagccgcctg ttgggggagg aaattgcttg tttcaattct tgaacatggt
     1141 gaagggtaaa tctctctctc accgggggtt gtgtgaatgc aaggcccttt accacgttgc
     1201 ttcttcctcc accaaccaag ccctttggaa tgggtcttgg cgagctctct ggggacaact
     1261 gtatatcgaa cctttgtgcc tgtgntccct caanggaaca cgatctccta ggtgatgtaa
     1321 ataaataccc atac
//
