LOCUS       sequence-160840-        1281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PHGDH
FEATURES             Location/Qualifiers
     source          1..1281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcccatagc ttctttcggg ctttgttagc agccggatcc ttagaagtgg aactggaagg
       61 cttcagtcac atgctgcttc cacgcttcca ggctgggcag caaggaggag atgcccatga
      121 cgtgccaggt ctccccatct gacaccagtg aagtctggta ggacagcagc cgcacgcctg
      181 cctctgccag gaggccaatc atggtaggca gcattgcagg gtcagaggtc tgagtccgga
      241 ataggagcag gggcaggtcc ctgcggagag gcacttctgg cctgaagaca gctccattga
      301 gcccctgcag tacaggtgta gtgccttgga ccaagcccac agcctggtaa ggggcgcctg
      361 ccagggccac ggccaggagg cattccccga agccttgctc ccctggtgca gcagggctgt
      421 gggaggtggt gacattgagg ccagcctctt tcaccagcag cttagcgttc accaagttca
      481 catccgcctg cttggaagcc tctttcagga ggccgacaat gactgcgggg cttaggcagt
      541 tcccagcatt cttcagggat gttccctgtg ttatcacctg gatggtccct ttgggggacc
      601 cagcccaggc tcgcatcagt gtccccagag cttctgccag accaatccaa ggcttggtgt
      661 gtggagagaa ggcactggta agggcctggg cattcacaac ccccgtgaga gatttcccct
      721 tcaccatgtc cacgaactga acagcaattt cctccccaca gcggctctga gcctccttgg
      781 tgctggcacc caggtgggga cagctgatga cattctcatg gtccaccaaa ggcccggtcc
      841 cgtggcggct cttccgtaaa cacgtccagt gcagccccgg cacactggcc agactgcagg
      901 gcccggagca ggcgccttcg tccacgatcc ctccacgggc acagttcacc acacgcaccc
      961 ccttctgcac tgggcaaagt gttgtcattc agcaagcctg tcgtgagggc aggagagagt
     1021 gtgcacagtg atgaaatcac agagaggcag atctctcagg gcagctgctg aacaccaaag
     1081 agcgagacct ctggggaaat gatgggtcat accctatagt cttcatccaa gactgcatcg
     1141 gtagcctacc cttctctcat ctgcagcaag aattccaggg tcttcattca gcccgtcatg
     1201 actctttcgn cantggcgct cttatnnnng nnnccctggg gnnngnnctg ncagnncagt
     1261 gagtatcatc tctctcaaca a
//
