LOCUS       sequence-160877-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mst2 SARAH domain for interaction and spectroscopy 
            studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Rawat SJ, Araiza-Olivera D, Arias-Romero LE, Villamar-Cruz O, 
            Prudnikova TY, Roder H, Chernoff J
  TITLE     H-ras Inhibits the Hippo Pathway by Promoting Mst1/Mst2 
            Heterodimerization.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1556-63. doi: 
            10.1016/j.cub.2016.04.027. Epub 2016 May 26.
  PUBMED    27238285
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.027"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1556-63"
COMMENT     Sequence Label: pGEX4T-Mst2 SARAH
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..24
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(264..286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(431..449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        517..621
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(840..859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctggaagttc tgttccaggg gcccctggga tccgactttg actttttgaa aaattggagt
       61 ttagaagaac tacagatgcg gttaaaagca ctggacccca tgatggaacg ggagatagaa
      121 gaacttcgtc agagatacac tgcgaaaaga cagcccattc tggatgcgat ggatgcaaag
      181 tgcgaattcc cgggtcgact cgagcggccg catcgtgact gactgacgat ctgcctcgcg
      241 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      301 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      361 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga
      421 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      481 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      841 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg gtcgccgcat
      901 acactattct cagaatgact tggtttgagt actcancagt cacagaaaaa gcatct
//
