LOCUS       sequence-160879-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tetracycline-inducible SH-tagged (streptavidin-binding 
            peptide and HA tag) version of Mst2. Used in conjunction with Flp-In
            cells to generate single copy transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Rawat SJ, Creasy CL, Peterson JR, Chernoff J
  TITLE     The tumor suppressor Mst1 promotes changes in the cellular redox 
            state by phosphorylation and inactivation of peroxiredoxin-1 
            protein.
  JOURNAL   J Biol Chem. 2013 Mar 22;288(12):8762-71. doi: 
            10.1074/jbc.M112.414524. Epub 2013 Feb 5.
  PUBMED    23386615
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.414524"; journalName: "J Biol Chem"; date: 
            "2013-03-22- 22"; volume: "288"; issue: "12"; pages: "8762-71"
COMMENT     Sequence Label: pcDNA5/FRT/TO/SH/GW-Mst2
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             82..105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             795..803
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tcggatncna ccatngctag ctggagccac ccgcagttcg agaaaggtgg aggttcnnga
       61 ggtggatcgg gaggtggatc gtggagccac ccgcagttcg aaaaagcggc cgatatcaca
      121 agtttgtaca aaaaagttgg catggagcag ccgccggcgc ntaagagtaa actaaaaaag
      181 ntgagtgaag acagtttgac taagcagcct gaagaagttt ttgatgtatt agagaagctt
      241 ggagaagggt cttatggaag tgtatttaaa gcaatacaca aggaatccgg tcaagttgtc
      301 gcaattaaac aagtacctgt tgaatcagat cttcaggaaa taatcaaaga aatttccata
      361 atgcagcaat gtgacagccc atatgttgta aagtactatg gcagttattt taagaataca
      421 gacctctgga ttgttatgga gtactgtggc gctggctctg tctcagacat aattagatta
      481 cgaaacaaga cattaataga agatgaaatt gcaaccattc ttaaatctac attgaaagga
      541 ctagaatatt tgcactttat gagaaaaata cacagagata taaaagctgg aaatattctc
      601 ctcaatacag aaggacatgc aaaattggca gattttggag tggctggtca gttaacagat
      661 acaatggcaa aacgcaatac tgtaatagga actccatttt ggatggctcc tgaggtgatt
      721 caagaaatag gctataactg tgtggccgac atctggtccc ttggcattac ttctatagaa
      781 atggatgaag gaaaacctcc ttatgctgat atacatccaa tgagggctat ttttatgatt
      841 cccacaaatc caccaccaac attcagaaag ccagaacttt ggtccgatga tttcaccgat
      901 tttgttaaaa agtgtttggt gaag
//
