LOCUS       sequence-160889-       12211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses direct fusion VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-Sad...
FEATURES             Location/Qualifiers
     source          1..12211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..284
                     /label=chicken beta-actin promoter
     intron          286..1303
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1227..1249
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1311..1330
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1370..1969
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1370..1389)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1866..1886
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2007..2595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2060..2080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2478..2489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2616..3115
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2651..2674)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3048..3067
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3139..3148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3148..3297
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3304..3324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3754..4125
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             4144..4713
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             4741..5439
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             5461..5481
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8662..8709
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     repeat_region   8899..8961
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9012..9032)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9012..9030)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9051..9067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9051..9067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9064..9086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9075..9091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9099..9105,9106..9123,9124..9129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9144..9165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9282..9299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9453..10041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9533..9552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10212..11003,11004..11072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10835..10854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11073..11177)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11203..11658)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11340..11361)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11552..11571
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11785..11807
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11799..11816
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11800..11816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11826..11845
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11826..11844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   11874..11908
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
       61 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      121 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      181 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg
      301 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      361 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      421 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      481 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      541 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      601 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      661 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
      721 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
      781 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
      841 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
      901 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
      961 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     1021 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     1081 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1141 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     1201 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     1261 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1321 ggttattgtg ctgtctcatc attttggcaa agaattcgcc accatgacca tgaccgagta
     1381 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     1441 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     1501 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     1561 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     1621 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     1681 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     1741 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     1801 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     1861 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     1921 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tttaaaccat
     1981 gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2041 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2101 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2161 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2221 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2281 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2341 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2401 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2461 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2521 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2581 gggccgcctc cccgcatcct gcaggtaatt ctaccgggta ggggaggcgc ttttcccaag
     2641 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     2701 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     2761 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     2821 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actaggctcg tgcagatgga
     2881 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     2941 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     3001 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     3061 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     3121 cagcccaagc ttgtcgacgc caccatggat gctttagacg attttgactt agatatgctt
     3181 ggttcagacg cgttagacga cttcgaccta gacatgttag gctcagatgc attggacgac
     3241 ttcgatttag atatgttggg ctccgatgcc ctagatgact ttgatctaga tatgctaggt
     3301 agtcccaaaa agaagaggaa agtgggatcc cagtatctgc ccgacacaga tgatagacac
     3361 cgaatcgaag agaaacgcaa gcgaacgtat gaaaccttca aatcgatcat gaagaaatcg
     3421 cccttctcgg gtccgaccga tcccaggccc ccaccgagaa ggattgcggt cccgtcccgc
     3481 tcgtcggcca gcgtgccgaa gcctgcgccg cagccctacc ccttcacgtc gagcctgagc
     3541 acaatcaatt atgacgagtt cccgacgatg gtgttcccct cgggacaaat ctcacaagcc
     3601 tcggcgctcg caccagcgcc tccccaagtc cttccgcaag cgcctgcccc agcgcctgca
     3661 ccggcaatgg tgtccgccct cgcacaggcc cctgcgcccg tccccgtgct cgcgcctgga
     3721 ccgccccagg cggtcgctcc accggctccg aagccgacgc aggccggaga gggaacactc
     3781 tccgaagcac ttcttcaact ccagtttgat gacgaggatc ttggagcact ccttggaaac
     3841 tcgacagacc ctgcggtgtt taccgacctc gcgtcagtag ataactccga atttcagcag
     3901 cttttgaacc agggtatccc ggtcgcgcca catacaacgg agcccatgtt gatggaatac
     3961 cccgaagcaa tcacgagact tgtgacggga gcgcagcggc ctcccgatcc cgcacccgca
     4021 cctttggggg cacctggcct ccctaacgga cttttgagcg gcgacgagga tttctcctcc
     4081 atcgccgata tggatttctc agccttgctg tcacagattt ccagcggctc tggcagcggc
     4141 agccgggatt ccagggaagg gatgtttttg ccgaagcctg aggccggctc cgctattagt
     4201 gacgtgtttg agggccgcga ggtgtgccag ccaaaacgaa tccggccatt tcatcctcca
     4261 ggaagtccat gggccaaccg cccactcccc gccagcctcg caccaacacc aaccggtcca
     4321 gtacatgagc cagtcgggtc actgaccccg gcaccagtcc ctcagccact ggatccagcg
     4381 cccgcagtga ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga gacgagccag
     4441 gctgtcaaag cccttcggga gatggccgat actgtgattc cccagaagga agaggctgca
     4501 atctgtggcc aaatggacct ttcccatccg cccccaaggg gccatctgga tgagctgaca
     4561 accacacttg agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg
     4621 aacgagattc tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc
     4681 acaggactgt ccatcttcga cacatctctg tttcatatgt caagcgctgc aggtgctacc
     4741 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     4801 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     4861 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     4921 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     4981 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     5041 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     5101 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     5161 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     5221 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     5281 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     5341 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     5401 tactgcgacc tccctagcaa actggggcac aagcttaatg gtggaggctc tggaatggcc
     5461 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccaagcg gaactacatc
     5521 ctgggcctgg ccatcggcat caccagcgtg ggctacggca tcatcgacta cgagacacgg
     5581 gacgtgatcg atgccggcgt gcggctgttc aaagaggcca acgtggaaaa caacgagggc
     5641 aggcggagca agagaggcgc cagaaggctg aagcggcgga ggcggcatag aatccagaga
     5701 gtgaagaagc tgctgttcga ctacaacctg ctgaccgacc acagcgagct gagcggcatc
     5761 aacccctacg aggccagagt gaagggcctg agccagaagc tgagcgagga agagttctct
     5821 gccgccctgc tgcacctggc caagagaaga ggcgtgcaca acgtgaacga ggtggaagag
     5881 gacaccggca acgagctgtc caccaaagag cagatcagcc ggaacagcaa ggccctggaa
     5941 gagaaatacg tggccgaact gcagctggaa cggctgaaga aagacggcga agtgcggggc
     6001 agcatcaaca gattcaagac cagcgactac gtgaaagaag ccaaacagct gctgaaggtg
     6061 cagaaggcct accaccagct ggaccagagc ttcatcgaca cctacatcga cctgctggaa
     6121 acccggcgga cctactatga gggacctggc gagggcagcc ccttcggctg gaaggacatc
     6181 aaagaatggt acgagatgct gatgggccac tgcacctact tccccgagga actgcggagc
     6241 gtgaagtacg cctacaacgc cgacctgtac aacgccctga acgacctgaa caatctcgtg
     6301 atcaccaggg acgagaacga gaagctggaa tattacgaga agttccagat catcgagaac
     6361 gtgttcaagc agaagaagaa gcccaccctg aagcagatcg ccaaagaaat cctcgtgaac
     6421 gaagaggata ttaagggcta cagagtgacc agcaccggca agcccgagtt caccaacctg
     6481 aaggtgtacc acgacatcaa ggacattacc gcccggaaag agattattga gaacgccgag
     6541 ctgctggatc agattgccaa gatcctgacc atctaccaga gcagcgagga catccaggaa
     6601 gaactgacca atctgaactc cgagctgacc caggaagaga tcgagcagat ctctaatctg
     6661 aagggctata ccggcaccca caacctgagc ctgaaggcca tcaacctgat cctggacgag
     6721 ctgtggcaca ccaacgacaa ccagatcgct atcttcaacc ggctgaagct ggtgcccaag
     6781 aaggtggacc tgtcccagca gaaagagatc cccaccaccc tggtggacga cttcatcctg
     6841 agccccgtcg tgaagagaag cttcatccag agcatcaaag tgatcaacgc catcatcaag
     6901 aagtacggcc tgcccaacga catcattatc gagctggccc gcgagaagaa ctccaaggac
     6961 gcccagaaaa tgatcaacga gatgcagaag cggaaccggc agaccaacga gcggatcgag
     7021 gaaatcatcc ggaccaccgg caaagagaac gccaagtacc tgatcgagaa gatcaagctg
     7081 cacgacatgc aggaaggcaa gtgcctgtac agcctggaag ccatccctct ggaagatctg
     7141 ctgaacaacc ccttcaacta tgaggtggac cacatcatcc ccagaagcgt gtccttcgac
     7201 aacagcttca acaacaaggt gctcgtgaag caggaagaag ccagcaagaa gggcaaccgg
     7261 accccattcc agtacctgag cagcagcgac agcaagatca gctacgaaac cttcaagaag
     7321 cacatcctga atctggccaa gggcaagggc agaatcagca agaccaagaa agagtatctg
     7381 ctggaagaac gggacatcaa caggttctcc gtgcagaaag acttcatcaa ccggaacctg
     7441 gtggatacca gatacgccac cagaggcctg atgaacctgc tgcggagcta cttcagagtg
     7501 aacaacctgg acgtgaaagt gaagtccatc aatggcggct tcaccagctt tctgcggcgg
     7561 aagtggaagt ttaagaaaga gcggaacaag gggtacaagc accacgccga ggacgccctg
     7621 atcattgcca acgccgattt catcttcaaa gagtggaaga aactggacaa ggccaaaaaa
     7681 gtgatggaaa accagatgtt cgaggaaaag caggccgaga gcatgcccga gatcgaaacc
     7741 gagcaggagt acaaagagat cttcatcacc ccccaccaga tcaagcacat taaggacttc
     7801 aaggactaca agtacagcca ccgggtggac aagaagccta atagagagct gattaacgac
     7861 accctgtact ccacccggaa ggacgacaag ggcaacaccc tgatcgtgaa caatctgaac
     7921 ggcctgtacg acaaggacaa tgacaagctg aaaaagctga tcaacaagag ccccgaaaag
     7981 ctgctgatgt accaccacga cccccagacc taccagaaac tgaagctgat tatggaacag
     8041 tacggcgacg agaagaatcc cctgtacaag tactacgagg aaaccgggaa ctacctgacc
     8101 aagtactcca aaaaggacaa cggccccgtg atcaagaaga ttaagtatta cggcaacaaa
     8161 ctgaacgccc atctggacat caccgacgac taccccaaca gcagaaacaa ggtcgtgaag
     8221 ctgtccctga agccctacag attcgacgtg tacctggaca atggcgtgta caagttcgtg
     8281 accgtgaaga atctggatgt gatcaaaaaa gaaaactact acgaagtgaa tagcaagtgc
     8341 tatgaggaag ctaagaagct gaagaagatc agcaaccagg ccgagtttat cgcctccttc
     8401 tacaacaacg atctgatcaa gatcaacggc gagctgtata gagtgatcgg cgtgaacaac
     8461 gacctgctga accggatcga agtgaacatg atcgacatca cctaccgcga gtacctggaa
     8521 aacatgaacg acaagaggcc ccccaggatc attaagacaa tcgcctccaa gacccagagc
     8581 attaagaagt acagcacaga cattctgggc aacctgtatg aagtgaaatc taagaagcac
     8641 cctcagatca tcaaaaaggg caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa
     8701 aagaaaaagt aactcgagct agttaaaagt tttgttactt tatagaagaa attttgagtt
     8761 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     8821 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     8881 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     8941 aatatgatta tctttctagg gttaatctag tatacgcgta tgcggccgct taattaatcc
     9001 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     9061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
    11221 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    11281 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    11341 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    11401 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    11461 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    11521 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    11581 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    11641 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    11701 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11761 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    11821 gcgcgtaata cgactcacta tagggcgaat tggggcgcgc cattctagat taaccctaga
    11881 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
    11941 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
    12001 gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa
    12061 aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga taattatatt
    12121 gttatttcat gttctactta cgtgataact tattatatat atattttctt gttatagata
    12181 tcaactagaa tgctagcatg ggcccagatc t
//
