LOCUS       sequence-160932-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 7Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-7Q
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(821..839)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      835..882
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        942..1046
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttncctctag aataattttg tttactttaa gaaggagata tacatatgca tcatcatcat
       61 caccatagcg gtgcaattag cggtgatagc ctgattagcc tggcaagcac cggtaaacgt
      121 gttagcatta aagatctgct ggatgagaaa gattttgaga tttgggccat taacgagcag
      181 accatgaaac tggaaagcgc aaaagttagc cgtgtttttt gcaccggtaa aaaactggtg
      241 tatatcctga aaacccgtct gggtcgtacc attaaagcaa ccgcaaatca tcgttttctg
      301 accattgatg gttggaaacg tctggatgaa ctgagcctga aagaacatat tgcactgcct
      361 cgtaaactgg aaagtagcag cctgcaactg agtccggaaa ttgaaaaact gagccagagc
      421 gatatctatt gggatagcat tgttagcatt accgaaaccg gtgttgaaga agtttttgat
      481 ctgaccgttc cgggtccgca caattttgtt gcaaatgata ttattgtgca caacgccacc
      541 ctggaaaaac tgatgaaagc atttgaaagc ctgaaaagct ttcagcagca gcagcaacag
      601 cagcctcctc cgcctccgcc tccaccaccc cctccacagc tgcctcagcc tccgccacag
      661 gcacagcctc tgctgccaca gccacaacca cccccaccac cgccaccacc tccgcctggt
      721 ccggcagttg cagaagaacc gctgcatcgt ccgtaataac tgcaggaagg ggatccggct
      781 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      841 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      901 tccggataac tacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgngtc gnccttattc
     1081 ccttttttgc ggcattttgc ctt
//
