LOCUS       sequence-160935-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 29Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-29Q
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(885..903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      899..946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattcccctc tagatatttt gtttacttta agaaggagat atacatatgc atcatcatca
       61 tcaccatagc ggtgcaatta gcggtgatag cctgattagc ctggcaagca ccggtaaacg
      121 tgttagcatt aaagatctgc tggatgagaa agattttgag atttgggcca ttaacgagca
      181 gaccatgaaa ctggaaagcg caaaagttag ccgtgttttt tgcaccggta aaaaactggt
      241 gtatatcctg aaaacccgtc tgggtcgtac cattaaagca accgcaaatc atcgttttct
      301 gaccattgat ggttggaaac gtctggatga actgagcctg aaagaacata ttgcactgcc
      361 tcgtaaactg gaaagtagca gcctgcaact gagtccggaa attgaaaaac tgagccagag
      421 cgatatctat tgggatagca ttgttagcat taccgaaacc ggtgttgaag aagtttttga
      481 tctgaccgtt ccgggtccgc acaattttgt tgcaaatgat attattgtgc acaacgccac
      541 cctggaaaaa ctgatgaaag catttgaaag cctgaaaagc tttcagcagc agcaacaaca
      601 gcagcaacag caacagcagc agcagcagca acaacagcag cagcaacagc aacaacaaca
      661 acaacagcag cctcctccgc ctccgccacc gcctccacct ccgcagctgc cgcagcctcc
      721 acctcaggca caaccgctgc tgcctcagcc gcagccacct cctccgccac caccgccacc
      781 gcctggtcca gcagttgcag aagaaccgct gcatcgtccg taactgcagg aaggggatcc
      841 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      901 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      961 tatatccgga taactacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1081 atgcttcaa
//
