LOCUS       sequence-160938-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 43Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-43Q
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(924..942)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctanaaatat tttgtttact ttaagaaagg agatatacat atgcatcatc atcctcacca
       61 tagcggtgca attagcggtg atagcctgat tagcctggca agcaccggta aacgtgttag
      121 cattaaagat ctgctggatg agaaagattt tgagatttgg gccattaacg agcagaccat
      181 gaaactggaa agcgcaaaag ttagccgtgt tttttgcacc ggtaaaaaac tggtgtatat
      241 cctgaaaacc cgtctgggtc gtaccattaa agcaaccgca aatcatcgtt ttctgaccat
      301 tgatggttgg aaacgtctgg atgaactgag cctgaaagaa catattgcac tgcctcgtaa
      361 actggaaagt agcagcctgc aactgagtcc ggaaattgaa aaactgagcc agagcgatat
      421 ctattgggat agcattgtta gcattaccga aaccggtgtt gaagaagttt ttgatctgac
      481 cgttccgggt ccgcacaatt ttgttgcaaa tgatattatt gtgcacaacg ccaccctgga
      541 aaaactgatg aaagcatttg aaagcctgaa aagctttcag cagcagcaac aacagcagca
      601 acagcaacag cagcagcagc agcaacaaca gcagcagcaa cagcaacaac aacaacaaca
      661 gcagcaacaa cagcaacagc agcaacaaca acagcaacag caacaaccgc ctccgcctcc
      721 gccacctcct cctccaccac agctgcctca gcctccgcct caggcacagc ctctgctgcc
      781 acagcctcag ccacctccac cgcctccacc tccacctccg ggtccggcag ttgcagaaga
      841 accgctgcat cgtccgtaat aactgcagga aggggatccg gctgctaaca aagcccgaaa
      901 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      961 taaacggnnc ttgaggggtt tttttgctga aaggaggaac tatatccggg ataactacgt
     1021 cangggggca cttttnnngg gaaatgnnnc nngnaanccc tanttggtta atttttccna
     1081 aa
//
