LOCUS       sequence-160940-         165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 165)
  AUTHORS   Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, 
            Garcia BA, Tuschl T, Stadtfeld M, Reinberg D
  TITLE     Co-repressor CBFA2T2 regulates pluripotency and germline 
            development.
  JOURNAL   Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.
  PUBMED    27281218
REFERENCE   2  (bases 1 to 165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-8- 8"; volume: "534"; issue: "7607"; pages:
            "387-90"
COMMENT     Sequence Label: mCBFKOgRNA5
FEATURES             Location/Qualifiers
     source          1..165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
       61 tatatcttgt ggaaaggacg aaacaccgct ggcccccagg attcataagt tttagagcta
      121 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaa
//
