LOCUS       sequence-160956-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the endoplasmic 
            reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV G-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..36)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(46..486)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKDLGTVLRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVWDKDGNGYISAADL
                     RHVMTNLGEKLTDEEVDEMIREADIDGEGQVNYEEFVQMMTAK"
     primer_bind     602..621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cccattcaga tcctcttctg agatgagttt ttgttctgcg gccgccttcg ctgtcatcat
       61 ttgtacaaac tcttcgtagt ttacctgacc ttctccatcg atatctgctt ccctgatcat
      121 ttcatcaacc tcttcatctg ttaacttctc tccaaggttt gtcatcacgt ggcgaaggtc
      181 tgctgcagag atgtagccat tgccatcctt atcccacaca cggaacgctt cgcgaatttc
      241 ttcttcactg tctgtgtctt tcatttttct tgccatcatt gtcaggaact cagggaagtc
      301 gattgtgcca ttaccgtcgg catctacttc attgatcatg tcctggagct ctgcttctgt
      361 ggggttctgc cccagagacc gcagcaccgt ccccagatcc ttggttgtta tcgtcccatc
      421 cccgtccttg tcaaataggg agaaagcctc tttaaattct gcgatctgct cttcagtcag
      481 ttggtcacgc gtgttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      541 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      601 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggatgt agccttcggg
      661 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
      721 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      781 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcaccct cgccggacac
      841 gctgaacttg tggccgt
//
