LOCUS       sequence-160957-         409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            T7 (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB427
FEATURES             Location/Qualifiers
     source          1..409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(308..340)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMXGGQQMG"
     promoter        complement(363..409)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gcccctcgag gcctcgaggc ctcgaggggc cgccatagtg
      181 actggatatg ttgtgtttta cngtattnng tagtctgttt tttatgcaaa atntaattta
      241 atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac anacttgtga
      301 taacagcacc catttgctgt ccaccantca tgctagccat gctcattcta gagtcccccg
      361 tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgc
//
