LOCUS       sequence-160977-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human ID2 with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 ID2-V5 puro
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(441..465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             467..508
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(603..623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 actgtcggga tcaacaagtt tgtacaaaaa agttggcatg aaagccttca gtcccgtgag
       61 gtccgttagg aaaaacagcc tgtcggacca cagcctgggc atctcccgga gcaaaacccc
      121 tgtggacgac ccgatgagcc tgctatacaa catgaacgac tgctactcca agctcaagga
      181 gctggtgccc agcatccccc agaacaagaa ggtgagcaag atggaaatcc tgcagcacgt
      241 catcgactac atcttggacc tgcagatcgc cctggactcg catcccacta ttgtcagcct
      301 gcatcaccag agacccgggc agaaccaggc gtccaggacg ccgctgacca ccctcaacac
      361 ggatatcagc atcctgtcct tgcaggcttc tgaattccct tctgagttaa tgtcaaatga
      421 cagcaaagca ctgtgtggct gcccaacttt cttgtacaaa gtggttggta agcctatccc
      481 taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt
      541 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
      601 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
      661 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
      721 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
//
