LOCUS       sequence-160978-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human ID2 with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 ID2-V5 puro
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(527..551)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(574..777)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(603..623)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca ttactacgta
       61 gaatcgagac cgaggagagg gttagggata ggcttaccaa ccactttgta caagaaagtt
      121 gggcagccac acagtgcttt gctgtcattt gacattaact cagaagggaa ttcagaagcc
      181 tgcaaggaca ggatgctgat atccgtgttg agggtggtca gcggcgtcct ggacgcctgg
      241 ttctgcccgg gtctctggtg atgcaggctg acaatagtgg gatgcgagtc cagggcgatc
      301 tgcaggtcca agatgtagtc gatgacgtgc tgcaggattt ccatcttgct caccttcttg
      361 ttctggggga tgctgggcac cagctccttg agcttggagt agcagtcgtt catgttgtat
      421 agcaggctca tcgggtcgtc cacaggggtt ttgctccggg agatgcccag gctgtggtcc
      481 gacaggctgt ttttcctaac ggacctcacg ggactgaagg ctttcatgcc aacttttttg
      541 tacaaacttg ttgatcccga cagttagcca gagagctctg cttatataga cctcccaccg
      601 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      661 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      721 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      781 ggtaatagcg atgactaata cgtagatgta ctgcca
//
