LOCUS       sequence-160984-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with C-terminal Venus tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS7
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     170..192
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     184..201
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     185..201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          254..826
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     834..853
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 tgaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
       61 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      121 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      181 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc tagaggatcc
      241 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
      301 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
      361 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
      421 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
      481 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
      541 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
      601 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
      661 gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc
      721 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
      781 gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
      841 gctggttatt gtgctgtctc atcattttgg caaagaattc gcggccgaa
//
